Effects of oxygen aeration on production of monoclonal antibody in immobilized hybridoma-cell bioreactor

Masamichi Kamihira; Hitoshi Yoshida; Shinji Iijima; Takeshi Kobayashi

doi:10.1016/0922-338X(90)90113-B

Effects of oxygen aeration on production of monoclonal antibody in immobilized hybridoma-cell bioreactor

Masamichi Kamihira, Hitoshi Yoshida, Shinji Iijima, Takeshi Kobayashi

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Continuous production of monoclonal antibody by hybridoma cells immobilized with alginate and urethane polymer was done using a fluidized bed bioreactor with direct bubbling. Increasing the dilution rate did not affect the antibody production rate under aeration with air. By changing the aeration gas to oxygen instead of air, the antibody production rate was increased 2-fold. The monoclonal antibody could be produced continuously for more than 40 d.

Original language	English
Pages (from-to)	311-312
Number of pages	2
Journal	Journal of Fermentation and Bioengineering
Volume	69
Issue number	5
DOIs	https://doi.org/10.1016/0922-338X(90)90113-B
Publication status	Published - 1990
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biotechnology
Applied Microbiology and Biotechnology

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10.1016/0922-338X(90)90113-B

Cite this

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title = "Effects of oxygen aeration on production of monoclonal antibody in immobilized hybridoma-cell bioreactor",

abstract = "Continuous production of monoclonal antibody by hybridoma cells immobilized with alginate and urethane polymer was done using a fluidized bed bioreactor with direct bubbling. Increasing the dilution rate did not affect the antibody production rate under aeration with air. By changing the aeration gas to oxygen instead of air, the antibody production rate was increased 2-fold. The monoclonal antibody could be produced continuously for more than 40 d.",

author = "Masamichi Kamihira and Hitoshi Yoshida and Shinji Iijima and Takeshi Kobayashi",

year = "1990",

doi = "10.1016/0922-338X(90)90113-B",

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AU - Kamihira, Masamichi

AU - Yoshida, Hitoshi

AU - Iijima, Shinji

AU - Kobayashi, Takeshi

PY - 1990

Y1 - 1990

N2 - Continuous production of monoclonal antibody by hybridoma cells immobilized with alginate and urethane polymer was done using a fluidized bed bioreactor with direct bubbling. Increasing the dilution rate did not affect the antibody production rate under aeration with air. By changing the aeration gas to oxygen instead of air, the antibody production rate was increased 2-fold. The monoclonal antibody could be produced continuously for more than 40 d.

AB - Continuous production of monoclonal antibody by hybridoma cells immobilized with alginate and urethane polymer was done using a fluidized bed bioreactor with direct bubbling. Increasing the dilution rate did not affect the antibody production rate under aeration with air. By changing the aeration gas to oxygen instead of air, the antibody production rate was increased 2-fold. The monoclonal antibody could be produced continuously for more than 40 d.

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IS - 5

ER -

Effects of oxygen aeration on production of monoclonal antibody in immobilized hybridoma-cell bioreactor

Abstract

All Science Journal Classification (ASJC) codes

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