Effects of oxygen aeration on production of monoclonal antibody in immobilized hybridoma-cell bioreactor

Masamichi Kamihira, Hitoshi Yoshida, Shinji Iijima, Takeshi Kobayashi

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Continuous production of monoclonal antibody by hybridoma cells immobilized with alginate and urethane polymer was done using a fluidized bed bioreactor with direct bubbling. Increasing the dilution rate did not affect the antibody production rate under aeration with air. By changing the aeration gas to oxygen instead of air, the antibody production rate was increased 2-fold. The monoclonal antibody could be produced continuously for more than 40 d.

Original languageEnglish
Pages (from-to)311-312
Number of pages2
JournalJournal of Fermentation and Bioengineering
Volume69
Issue number5
DOIs
Publication statusPublished - 1990
Externally publishedYes

Fingerprint

Immobilized Cells
Monoclonal antibodies
Hybridomas
Bioreactors
Antibody Formation
Air
Monoclonal Antibodies
Oxygen
Antibodies
Urethane
Polymers
Gases
Alginate
Fluidized beds
Dilution
alginic acid

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Effects of oxygen aeration on production of monoclonal antibody in immobilized hybridoma-cell bioreactor. / Kamihira, Masamichi; Yoshida, Hitoshi; Iijima, Shinji; Kobayashi, Takeshi.

In: Journal of Fermentation and Bioengineering, Vol. 69, No. 5, 1990, p. 311-312.

Research output: Contribution to journalArticle

@article{8cdc3cc809c443b19fb610aae80411f0,
title = "Effects of oxygen aeration on production of monoclonal antibody in immobilized hybridoma-cell bioreactor",
abstract = "Continuous production of monoclonal antibody by hybridoma cells immobilized with alginate and urethane polymer was done using a fluidized bed bioreactor with direct bubbling. Increasing the dilution rate did not affect the antibody production rate under aeration with air. By changing the aeration gas to oxygen instead of air, the antibody production rate was increased 2-fold. The monoclonal antibody could be produced continuously for more than 40 d.",
author = "Masamichi Kamihira and Hitoshi Yoshida and Shinji Iijima and Takeshi Kobayashi",
year = "1990",
doi = "10.1016/0922-338X(90)90113-B",
language = "English",
volume = "69",
pages = "311--312",
journal = "Journal of Bioscience and Bioengineering",
issn = "1389-1723",
publisher = "The Society for Biotechnology, Japan",
number = "5",

}

TY - JOUR

T1 - Effects of oxygen aeration on production of monoclonal antibody in immobilized hybridoma-cell bioreactor

AU - Kamihira, Masamichi

AU - Yoshida, Hitoshi

AU - Iijima, Shinji

AU - Kobayashi, Takeshi

PY - 1990

Y1 - 1990

N2 - Continuous production of monoclonal antibody by hybridoma cells immobilized with alginate and urethane polymer was done using a fluidized bed bioreactor with direct bubbling. Increasing the dilution rate did not affect the antibody production rate under aeration with air. By changing the aeration gas to oxygen instead of air, the antibody production rate was increased 2-fold. The monoclonal antibody could be produced continuously for more than 40 d.

AB - Continuous production of monoclonal antibody by hybridoma cells immobilized with alginate and urethane polymer was done using a fluidized bed bioreactor with direct bubbling. Increasing the dilution rate did not affect the antibody production rate under aeration with air. By changing the aeration gas to oxygen instead of air, the antibody production rate was increased 2-fold. The monoclonal antibody could be produced continuously for more than 40 d.

UR - http://www.scopus.com/inward/record.url?scp=0025102223&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025102223&partnerID=8YFLogxK

U2 - 10.1016/0922-338X(90)90113-B

DO - 10.1016/0922-338X(90)90113-B

M3 - Article

AN - SCOPUS:0025102223

VL - 69

SP - 311

EP - 312

JO - Journal of Bioscience and Bioengineering

JF - Journal of Bioscience and Bioengineering

SN - 1389-1723

IS - 5

ER -