Effects of positive pressure in odontogenic keratocysts

S. Oka, Y. Kubota, T. Yamashiro, S. Ogata, T. Ninomiya, S. Ito, K. Shirasuna

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Intracystic fluid pressure is thought to be involved in odontogenic cyst growth. In this study, we investigated the effects of positive pressure on the expression of interleukin-1α (IL-1α), matrix metalloproteinases (MMPs), and prostaglandin E2 (PGE2) in odontogenic keratocysts to determine whether this pressure stimulates inflammatory cytokine production and signaling of osteoclastogenic events. Positive pressure enhanced the expression of IL-1α mRNA and protein in odontogenic keratocyst epithelial cells, and increased the secretion of MMP-1, MMP-2, MMP-3, and PGE2 in a co-culture of odontogenic keratocyst fibroblasts and the epithelial cells. The pressure-induced secretions were inhibited by an interleukin-1 receptor antagonist. Recombinant human interleukin-1α (rhIL-1α) increased the secretion of MMP-1, MMP-2, MMP-3, and PGE 2 in the fibroblasts. Furthermore, in the fibroblasts, rhIL-1α enhanced the expression of macrophage colony-stimulating factor (M-CSF) mRNA, and rhIL-1α-induced PGE2 increased the expression of nuclear factor κB ligand (RANKL) mRNA. Thus, positive pressure may play a crucial role in odontogenic keratocyst growth via stimulating the expression of IL-1α in epithelial cells.

Original languageEnglish
Pages (from-to)913-918
Number of pages6
JournalJournal of Dental Research
Volume84
Issue number10
DOIs
Publication statusPublished - Oct 1 2005

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Odontogenic Cysts
Interleukin-1
Pressure
Dinoprostone
Matrix Metalloproteinase 3
Matrix Metalloproteinase 1
Fibroblasts
Epithelial Cells
Matrix Metalloproteinase 2
Messenger RNA
Macrophage Colony-Stimulating Factor
Interleukin-1 Receptors
Growth
Coculture Techniques
Prostaglandins E
Matrix Metalloproteinases
Cytokines
Ligands

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

Cite this

Oka, S., Kubota, Y., Yamashiro, T., Ogata, S., Ninomiya, T., Ito, S., & Shirasuna, K. (2005). Effects of positive pressure in odontogenic keratocysts. Journal of Dental Research, 84(10), 913-918. https://doi.org/10.1177/154405910508401008

Effects of positive pressure in odontogenic keratocysts. / Oka, S.; Kubota, Y.; Yamashiro, T.; Ogata, S.; Ninomiya, T.; Ito, S.; Shirasuna, K.

In: Journal of Dental Research, Vol. 84, No. 10, 01.10.2005, p. 913-918.

Research output: Contribution to journalArticle

Oka, S, Kubota, Y, Yamashiro, T, Ogata, S, Ninomiya, T, Ito, S & Shirasuna, K 2005, 'Effects of positive pressure in odontogenic keratocysts', Journal of Dental Research, vol. 84, no. 10, pp. 913-918. https://doi.org/10.1177/154405910508401008
Oka S, Kubota Y, Yamashiro T, Ogata S, Ninomiya T, Ito S et al. Effects of positive pressure in odontogenic keratocysts. Journal of Dental Research. 2005 Oct 1;84(10):913-918. https://doi.org/10.1177/154405910508401008
Oka, S. ; Kubota, Y. ; Yamashiro, T. ; Ogata, S. ; Ninomiya, T. ; Ito, S. ; Shirasuna, K. / Effects of positive pressure in odontogenic keratocysts. In: Journal of Dental Research. 2005 ; Vol. 84, No. 10. pp. 913-918.
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