Effects of pretreatment on the microbial community and l-lactic acid production in vinasse fermentation

Jianguo Liu, Qunhui Wang, Shuang Wang, Xiaohong Sun, Hongzhi Ma, Yukihiro Tushiro

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Microwave-alkali and steam-alkali coupled pretreatments were carried out to improve the yield and optical purity of l-lactic acid produced using vinasse fermentation. Lactobacillus casei was inoculated into the system to initiate fermentation. Polymerase chain reaction denaturing gradient gel electrophoresis was used to analyze the microbial community during fermentation with and without the pretreatments. The original bacterial genus in vinasse was essentially inactivated, whereas L. casei became the dominant genus after 24. h of fermentation. The system subjected to microwave-alkali coupled pretreatment released more reducing sugars and produced more lactic acid (up to 30.32. g/L), which is twice that without pretreatment. In addition, the proportion of lactic acid in the organic acids also increased. The optical purity of the l-lactic acid produced under the microwave-alkali coupled pretreatment reached 91%, which is 2% higher than that under the steam-alkali coupled pretreatment and 7% higher than that under the control conditions. Therefore, the microwave-alkali coupled pretreatment is an effective method for the highly efficient bioconversion of vinasse into bioenergy.

Original languageEnglish
Pages (from-to)260-265
Number of pages6
JournalJournal of Biotechnology
Volume164
Issue number2
DOIs
Publication statusPublished - Dec 5 2012

Fingerprint

Alkalies
Lactic acid
Fermentation
Lactic Acid
Microwaves
Lactobacillus casei
Steam
Bioconversion
Organic acids
Polymerase chain reaction
Electrophoresis
Sugars
Denaturing Gradient Gel Electrophoresis
Gels
Polymerase Chain Reaction
Acids

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Effects of pretreatment on the microbial community and l-lactic acid production in vinasse fermentation. / Liu, Jianguo; Wang, Qunhui; Wang, Shuang; Sun, Xiaohong; Ma, Hongzhi; Tushiro, Yukihiro.

In: Journal of Biotechnology, Vol. 164, No. 2, 05.12.2012, p. 260-265.

Research output: Contribution to journalArticle

Liu, Jianguo ; Wang, Qunhui ; Wang, Shuang ; Sun, Xiaohong ; Ma, Hongzhi ; Tushiro, Yukihiro. / Effects of pretreatment on the microbial community and l-lactic acid production in vinasse fermentation. In: Journal of Biotechnology. 2012 ; Vol. 164, No. 2. pp. 260-265.
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