Effects of substance P and calcitonin gene-related peptide on axonal transport in isolated and cultured adult mouse dorsal root ganglion neurons

Hiromi Hiruma, Ayako Saito, Takafumi Ichikawa, Yoriko Kiriyama, Sumio Hoka, Tatsumi Kusakabe, Hirosuke Kobayashi, Tadashi Kawakami

Research output: Contribution to journalArticle

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Abstract

Substance P and calcitonin gene-related peptide (CGRP) released from primary sensory neurons are known to play important roles in nociception and nociceptive transmission. In the present study, we attempted to clarify the roles of these neuropeptides in the regulation of axonal transport in sensory neurons. Cells were isolated from adult mouse dorsal root ganglia and cultured in F-12 medium containing fetal bovine serum for 48 h until their neurites were grown. These isolated and cultured DRG cells were mostly (>98%) small (diameter <25 μm) and medium (diameter, 25-40 μm) in size, and were immunoreactive for substance P and CGRP (85.9 and 66.0% of total cells, respectively). Video-enhanced microscopy was applied to observe particles transported within neurites. Application of substance P (100 nM) decreased the number of particles transported in both anterograde and retrograde directions in each of DRG neurons tested (n=5). The instantaneous velocities of individual particles transported in anterograde and retrograde directions were also reduced by substance P. In contrast, α-CGRP (100 nM) increased the number of particles transported in both directions in each of DRG neurons tested (n=5), and also increased the instantaneous velocities of particles transported bidirectionally. Application of β-CGRP (100-1000 nM) did not elicit any effect on axonal transport. Therefore, axonal transport in sensory neurons seems to be modulated by substance P and α-CGRP, both of which can be derived from its own and adjacent sensory neurons. (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)184-191
Number of pages8
JournalBrain Research
Volume883
Issue number2
DOIs
Publication statusPublished - Nov 17 2000

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Axonal Transport
Calcitonin Gene-Related Peptide
Spinal Ganglia
Substance P
Sensory Receptor Cells
Diagnosis-Related Groups
Neurons
Neurites
Video Microscopy
Nociception
Neuropeptides
Cultured Cells
Serum
Direction compound

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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Effects of substance P and calcitonin gene-related peptide on axonal transport in isolated and cultured adult mouse dorsal root ganglion neurons. / Hiruma, Hiromi; Saito, Ayako; Ichikawa, Takafumi; Kiriyama, Yoriko; Hoka, Sumio; Kusakabe, Tatsumi; Kobayashi, Hirosuke; Kawakami, Tadashi.

In: Brain Research, Vol. 883, No. 2, 17.11.2000, p. 184-191.

Research output: Contribution to journalArticle

Hiruma, H, Saito, A, Ichikawa, T, Kiriyama, Y, Hoka, S, Kusakabe, T, Kobayashi, H & Kawakami, T 2000, 'Effects of substance P and calcitonin gene-related peptide on axonal transport in isolated and cultured adult mouse dorsal root ganglion neurons', Brain Research, vol. 883, no. 2, pp. 184-191. https://doi.org/10.1016/S0006-8993(00)02892-4
Hiruma, Hiromi ; Saito, Ayako ; Ichikawa, Takafumi ; Kiriyama, Yoriko ; Hoka, Sumio ; Kusakabe, Tatsumi ; Kobayashi, Hirosuke ; Kawakami, Tadashi. / Effects of substance P and calcitonin gene-related peptide on axonal transport in isolated and cultured adult mouse dorsal root ganglion neurons. In: Brain Research. 2000 ; Vol. 883, No. 2. pp. 184-191.
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