Effects of synthetic kisspeptin peptides and GnRH analogue on oocyte growth and circulating sex steroids in prepubertal female chub mackerel (Scomber japonicus)

Sethu Selvaraj, Hirofumi Ohga, Mitsuo Nyuji, Hajime Kitano, Naoki Nagano, Akihiko Yamaguchi, Michiya Matsuyama

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

In recent years, kisspeptin peptides, encoded by kiss genes have been used to manipulate reproductive processes in farmed animals, including fish. Our previous studies demonstrated that the chub mackerel brain expresses kiss1 and kiss2 and intramuscular injection of synthetic Kiss1 pentadecapeptide (Kiss1-15) but not Kiss2 dodecapeptide (Kiss2-12) accelerates spermatogenesis in prepubertal male chub mackerel (Scomber japonicus). In the present study, we evaluated their effects in prepubertal female chub mackerel. The gonadosomatic index (GSI) values of experimental fish did not show any significant changes. Condition factor (CF) values increased significantly in Kiss1-15 treated fish, in comparison with control and GnRH analogue (GnRHa) injected fish. Histologically, only perinucleolar oocytes were found in all experimental fish. However, Kiss and GnRHa treated fish showed a significant increase in the perinucleolar oocyte diameter, in comparison with the control fish. Gene expression analyses revealed decreased expression of gnrh1 in the telencephalon-preoptic region of the brain of Kiss2-12 and GnRHa injected fish, in comparison with control fish. In contrast, GnRHa injected fish exhibited higher levels of fshβ in the pituitary, with no changes in the levels of lhβ among different treatments. Levels of circulating sex steroids, testosterone, and estradiol-17β were significantly higher in Kiss1-15 injected fish, in comparison with control fish. These results indicate that synthetic kisspeptin peptides and GnRHa can induce oocyte growth in prepubertal female chub mackerel.

Original languageEnglish
Pages (from-to)1866-1877
Number of pages12
JournalAquaculture Research
Volume46
Issue number8
DOIs
Publication statusPublished - Aug 1 2015

Fingerprint

Scomber japonicus
synthetic peptides
steroid
peptide
steroids
oocytes
gender
fish
effect
brain
condition factor
gonadosomatic index
testosterone
intramuscular injection
spermatogenesis
gene expression
estradiol

All Science Journal Classification (ASJC) codes

  • Aquatic Science

Cite this

Effects of synthetic kisspeptin peptides and GnRH analogue on oocyte growth and circulating sex steroids in prepubertal female chub mackerel (Scomber japonicus). / Selvaraj, Sethu; Ohga, Hirofumi; Nyuji, Mitsuo; Kitano, Hajime; Nagano, Naoki; Yamaguchi, Akihiko; Matsuyama, Michiya.

In: Aquaculture Research, Vol. 46, No. 8, 01.08.2015, p. 1866-1877.

Research output: Contribution to journalArticle

@article{0314fc9c4ce84c218d6d8226fe423eaa,
title = "Effects of synthetic kisspeptin peptides and GnRH analogue on oocyte growth and circulating sex steroids in prepubertal female chub mackerel (Scomber japonicus)",
abstract = "In recent years, kisspeptin peptides, encoded by kiss genes have been used to manipulate reproductive processes in farmed animals, including fish. Our previous studies demonstrated that the chub mackerel brain expresses kiss1 and kiss2 and intramuscular injection of synthetic Kiss1 pentadecapeptide (Kiss1-15) but not Kiss2 dodecapeptide (Kiss2-12) accelerates spermatogenesis in prepubertal male chub mackerel (Scomber japonicus). In the present study, we evaluated their effects in prepubertal female chub mackerel. The gonadosomatic index (GSI) values of experimental fish did not show any significant changes. Condition factor (CF) values increased significantly in Kiss1-15 treated fish, in comparison with control and GnRH analogue (GnRHa) injected fish. Histologically, only perinucleolar oocytes were found in all experimental fish. However, Kiss and GnRHa treated fish showed a significant increase in the perinucleolar oocyte diameter, in comparison with the control fish. Gene expression analyses revealed decreased expression of gnrh1 in the telencephalon-preoptic region of the brain of Kiss2-12 and GnRHa injected fish, in comparison with control fish. In contrast, GnRHa injected fish exhibited higher levels of fshβ in the pituitary, with no changes in the levels of lhβ among different treatments. Levels of circulating sex steroids, testosterone, and estradiol-17β were significantly higher in Kiss1-15 injected fish, in comparison with control fish. These results indicate that synthetic kisspeptin peptides and GnRHa can induce oocyte growth in prepubertal female chub mackerel.",
author = "Sethu Selvaraj and Hirofumi Ohga and Mitsuo Nyuji and Hajime Kitano and Naoki Nagano and Akihiko Yamaguchi and Michiya Matsuyama",
year = "2015",
month = "8",
day = "1",
doi = "10.1111/are.12342",
language = "English",
volume = "46",
pages = "1866--1877",
journal = "Aquaculture Research",
issn = "1355-557X",
publisher = "Wiley-Blackwell",
number = "8",

}

TY - JOUR

T1 - Effects of synthetic kisspeptin peptides and GnRH analogue on oocyte growth and circulating sex steroids in prepubertal female chub mackerel (Scomber japonicus)

AU - Selvaraj, Sethu

AU - Ohga, Hirofumi

AU - Nyuji, Mitsuo

AU - Kitano, Hajime

AU - Nagano, Naoki

AU - Yamaguchi, Akihiko

AU - Matsuyama, Michiya

PY - 2015/8/1

Y1 - 2015/8/1

N2 - In recent years, kisspeptin peptides, encoded by kiss genes have been used to manipulate reproductive processes in farmed animals, including fish. Our previous studies demonstrated that the chub mackerel brain expresses kiss1 and kiss2 and intramuscular injection of synthetic Kiss1 pentadecapeptide (Kiss1-15) but not Kiss2 dodecapeptide (Kiss2-12) accelerates spermatogenesis in prepubertal male chub mackerel (Scomber japonicus). In the present study, we evaluated their effects in prepubertal female chub mackerel. The gonadosomatic index (GSI) values of experimental fish did not show any significant changes. Condition factor (CF) values increased significantly in Kiss1-15 treated fish, in comparison with control and GnRH analogue (GnRHa) injected fish. Histologically, only perinucleolar oocytes were found in all experimental fish. However, Kiss and GnRHa treated fish showed a significant increase in the perinucleolar oocyte diameter, in comparison with the control fish. Gene expression analyses revealed decreased expression of gnrh1 in the telencephalon-preoptic region of the brain of Kiss2-12 and GnRHa injected fish, in comparison with control fish. In contrast, GnRHa injected fish exhibited higher levels of fshβ in the pituitary, with no changes in the levels of lhβ among different treatments. Levels of circulating sex steroids, testosterone, and estradiol-17β were significantly higher in Kiss1-15 injected fish, in comparison with control fish. These results indicate that synthetic kisspeptin peptides and GnRHa can induce oocyte growth in prepubertal female chub mackerel.

AB - In recent years, kisspeptin peptides, encoded by kiss genes have been used to manipulate reproductive processes in farmed animals, including fish. Our previous studies demonstrated that the chub mackerel brain expresses kiss1 and kiss2 and intramuscular injection of synthetic Kiss1 pentadecapeptide (Kiss1-15) but not Kiss2 dodecapeptide (Kiss2-12) accelerates spermatogenesis in prepubertal male chub mackerel (Scomber japonicus). In the present study, we evaluated their effects in prepubertal female chub mackerel. The gonadosomatic index (GSI) values of experimental fish did not show any significant changes. Condition factor (CF) values increased significantly in Kiss1-15 treated fish, in comparison with control and GnRH analogue (GnRHa) injected fish. Histologically, only perinucleolar oocytes were found in all experimental fish. However, Kiss and GnRHa treated fish showed a significant increase in the perinucleolar oocyte diameter, in comparison with the control fish. Gene expression analyses revealed decreased expression of gnrh1 in the telencephalon-preoptic region of the brain of Kiss2-12 and GnRHa injected fish, in comparison with control fish. In contrast, GnRHa injected fish exhibited higher levels of fshβ in the pituitary, with no changes in the levels of lhβ among different treatments. Levels of circulating sex steroids, testosterone, and estradiol-17β were significantly higher in Kiss1-15 injected fish, in comparison with control fish. These results indicate that synthetic kisspeptin peptides and GnRHa can induce oocyte growth in prepubertal female chub mackerel.

UR - http://www.scopus.com/inward/record.url?scp=84936169067&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84936169067&partnerID=8YFLogxK

U2 - 10.1111/are.12342

DO - 10.1111/are.12342

M3 - Article

AN - SCOPUS:84936169067

VL - 46

SP - 1866

EP - 1877

JO - Aquaculture Research

JF - Aquaculture Research

SN - 1355-557X

IS - 8

ER -