Effects of temperature and nutrient concentration on the structural characteristics and removal of vegetable-associated Pseudomonas biofilm

Daisuke Hamanaka, Mami Onishi, Takuma Genkawa, Fumihiko Tanaka, Toshitaka Uchino

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The development of vegetable-associated Pseudomonas biofilms on polystyrene surfaces with different nutrient concentrations (trypticase soy broth; TSB, and 1:20 diluted TSB) was evaluated at 5 and 30 °C for up to 5 days. Biofilm structural characteristics were observed by confocal scanning laser microscopy (CSLM) before and after water-removal treatment. Before removal treatment, the bacterial counts of biofilm at 30 °C were 0.5-1 logs greater than that at 5 °C throughout incubation. However, the detected cell counts of biofilm developed with diluted TSB were greater than that with TSB at both 5 and 30 °C. Although the difference in the structure of developed biofilm between TSB and diluted TSB was observed at 30 °C, it was not clear that the removal treatment affected Pseudomonas biofilm structure.

Original languageEnglish
Pages (from-to)165-170
Number of pages6
JournalFood Control
Volume24
Issue number1-2
DOIs
Publication statusPublished - Mar 1 2012

Fingerprint

Biofilms
Pseudomonas
Vegetables
biofilm
nutrient content
vegetables
Food
Temperature
temperature
Bacterial Load
Water Purification
Polystyrenes
polystyrenes
confocal laser scanning microscopy
Confocal Microscopy
plate count
Cell Count
water
cells

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science

Cite this

Effects of temperature and nutrient concentration on the structural characteristics and removal of vegetable-associated Pseudomonas biofilm. / Hamanaka, Daisuke; Onishi, Mami; Genkawa, Takuma; Tanaka, Fumihiko; Uchino, Toshitaka.

In: Food Control, Vol. 24, No. 1-2, 01.03.2012, p. 165-170.

Research output: Contribution to journalArticle

Hamanaka, Daisuke ; Onishi, Mami ; Genkawa, Takuma ; Tanaka, Fumihiko ; Uchino, Toshitaka. / Effects of temperature and nutrient concentration on the structural characteristics and removal of vegetable-associated Pseudomonas biofilm. In: Food Control. 2012 ; Vol. 24, No. 1-2. pp. 165-170.
@article{d867e9a64144400ebbff393b24d6d08b,
title = "Effects of temperature and nutrient concentration on the structural characteristics and removal of vegetable-associated Pseudomonas biofilm",
abstract = "The development of vegetable-associated Pseudomonas biofilms on polystyrene surfaces with different nutrient concentrations (trypticase soy broth; TSB, and 1:20 diluted TSB) was evaluated at 5 and 30 °C for up to 5 days. Biofilm structural characteristics were observed by confocal scanning laser microscopy (CSLM) before and after water-removal treatment. Before removal treatment, the bacterial counts of biofilm at 30 °C were 0.5-1 logs greater than that at 5 °C throughout incubation. However, the detected cell counts of biofilm developed with diluted TSB were greater than that with TSB at both 5 and 30 °C. Although the difference in the structure of developed biofilm between TSB and diluted TSB was observed at 30 °C, it was not clear that the removal treatment affected Pseudomonas biofilm structure.",
author = "Daisuke Hamanaka and Mami Onishi and Takuma Genkawa and Fumihiko Tanaka and Toshitaka Uchino",
year = "2012",
month = "3",
day = "1",
doi = "10.1016/j.foodcont.2011.09.021",
language = "English",
volume = "24",
pages = "165--170",
journal = "Food Control",
issn = "0956-7135",
publisher = "Elsevier BV",
number = "1-2",

}

TY - JOUR

T1 - Effects of temperature and nutrient concentration on the structural characteristics and removal of vegetable-associated Pseudomonas biofilm

AU - Hamanaka, Daisuke

AU - Onishi, Mami

AU - Genkawa, Takuma

AU - Tanaka, Fumihiko

AU - Uchino, Toshitaka

PY - 2012/3/1

Y1 - 2012/3/1

N2 - The development of vegetable-associated Pseudomonas biofilms on polystyrene surfaces with different nutrient concentrations (trypticase soy broth; TSB, and 1:20 diluted TSB) was evaluated at 5 and 30 °C for up to 5 days. Biofilm structural characteristics were observed by confocal scanning laser microscopy (CSLM) before and after water-removal treatment. Before removal treatment, the bacterial counts of biofilm at 30 °C were 0.5-1 logs greater than that at 5 °C throughout incubation. However, the detected cell counts of biofilm developed with diluted TSB were greater than that with TSB at both 5 and 30 °C. Although the difference in the structure of developed biofilm between TSB and diluted TSB was observed at 30 °C, it was not clear that the removal treatment affected Pseudomonas biofilm structure.

AB - The development of vegetable-associated Pseudomonas biofilms on polystyrene surfaces with different nutrient concentrations (trypticase soy broth; TSB, and 1:20 diluted TSB) was evaluated at 5 and 30 °C for up to 5 days. Biofilm structural characteristics were observed by confocal scanning laser microscopy (CSLM) before and after water-removal treatment. Before removal treatment, the bacterial counts of biofilm at 30 °C were 0.5-1 logs greater than that at 5 °C throughout incubation. However, the detected cell counts of biofilm developed with diluted TSB were greater than that with TSB at both 5 and 30 °C. Although the difference in the structure of developed biofilm between TSB and diluted TSB was observed at 30 °C, it was not clear that the removal treatment affected Pseudomonas biofilm structure.

UR - http://www.scopus.com/inward/record.url?scp=80055083801&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80055083801&partnerID=8YFLogxK

U2 - 10.1016/j.foodcont.2011.09.021

DO - 10.1016/j.foodcont.2011.09.021

M3 - Article

AN - SCOPUS:80055083801

VL - 24

SP - 165

EP - 170

JO - Food Control

JF - Food Control

SN - 0956-7135

IS - 1-2

ER -