Effects of the opiates on the paraventricular nucleus in genetically polydipsic mice

Itsugi Nagatomo, Toshihiko Katafuchi, Kiyomi Koizumi

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The inbred mice, STR/N, are known to possess extreme polydipsia with no known abnormality in vasopressin system and the kidney function. Our previous studies indicate that the opiate antagonists given intracerebroventricularly strongly attenuated spontaneous drinking. To determine the site(s) of action, the present study was undertaken. Microinjections of naltrexone methobromide and a selective κ-receptor antagonist, nor-binaltorphimine (nor-BNI), into the paraventricular nucleus of the hypothalamus (PVN) greatly attenuated drinking of the STR/N for 0.5 to 16 h after injections, while in the two control groups, non-polydipsic STR/1N and Swiss/Webster strains, drinking was not affected by the injections. Food intake was not much altered in all groups. Studies of PVN neurons in vitro (n = > 160 for each group) showed that basal firing rates and patterns were similar in the STR/N and the control groups. Morphine added to the medium inhibited some but excited none in all strains tested. The threshold for the inhibitory action was higher in the polydipsic STR/N mice (10-8 M), compared to that in the control, S/W mice (10-9 M). Further, a proportion of neurons inhibited by morphine in the PVN was significantly smaller (P < 0.01) in the STR/N (41.7%), compared to that in the control (64.9%). Dynorphin had very similar effect to that of morphine, but the proportion of cells inhibited was 25.4% in the STR/N, and 70.4% in the S/W. Prior applications of naloxone to the medium prevented the action of both morphine and dynorphin. Under the synaptic blockade (in a low Ca2+ and high Mg2+ medium) the inhibitory effect of the opiates persisted. We concluded that the PVN is at least one of the possible sites where the opiates are acting to cause the polydipsia in the STR/N mice.

Original languageEnglish
Pages (from-to)23-32
Number of pages10
JournalBrain Research
Volume598
Issue number1-2
DOIs
Publication statusPublished - Dec 11 1992

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Opiate Alkaloids
Paraventricular Hypothalamic Nucleus
Morphine
Hypothalamus
Drinking
Polydipsia
Dynorphins
Neurons
Control Groups
Injections
Microinjections
Naloxone
Vasopressins
Eating
Kidney

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Effects of the opiates on the paraventricular nucleus in genetically polydipsic mice. / Nagatomo, Itsugi; Katafuchi, Toshihiko; Koizumi, Kiyomi.

In: Brain Research, Vol. 598, No. 1-2, 11.12.1992, p. 23-32.

Research output: Contribution to journalArticle

Nagatomo, Itsugi ; Katafuchi, Toshihiko ; Koizumi, Kiyomi. / Effects of the opiates on the paraventricular nucleus in genetically polydipsic mice. In: Brain Research. 1992 ; Vol. 598, No. 1-2. pp. 23-32.
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