Abstract
The objective of this study was to identify the mechanism by which mandibular condyle chondrocytes regulate the extracellular matrix. Primary rabbit condylar chondrocytes were isolated, cultured, and treated with transforming growth factor beta 1 (TGF-β1). Cells were then assayed for the following: urokinase-type plasminogen activator (uPA) and its inhibitor (PAI-1), collagen types I and II, β1 integrin expression, and proliferative activity. TGF-β1 induced synthesis of collagen type II, αVβ1 integrin, and PAI-1. TGF-β1 induced the growth of chondrocytes and suppressed the synthesis of uPA. Chondrocyte regulation of the extracellular matrix is mediated by TGF-β1. Synthesis of collagen type II, αVβ1 integrin, and PAI-1 is induced, while uPA is suppressed. Also, TGF-β1 induces cellular growth.
| Original language | English |
|---|---|
| Pages (from-to) | 470-475 |
| Number of pages | 6 |
| Journal | International Journal of Oral and Maxillofacial Surgery |
| Volume | 43 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Apr 1 2014 |
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All Science Journal Classification (ASJC) codes
- Surgery
- Oral Surgery
- Otorhinolaryngology
Cite this
Effects of transforming growth factor beta 1 on the plasminogen activation system, collagen and integrin synthesis, and proliferation of rabbit mandibular condylar chondrocytes. / Ishibashi, H.; Nariai, Y.; Kanno, T.; Onimaru, M.; Sekine, J.
In: International Journal of Oral and Maxillofacial Surgery, Vol. 43, No. 4, 01.04.2014, p. 470-475.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effects of transforming growth factor beta 1 on the plasminogen activation system, collagen and integrin synthesis, and proliferation of rabbit mandibular condylar chondrocytes
AU - Ishibashi, H.
AU - Nariai, Y.
AU - Kanno, T.
AU - Onimaru, M.
AU - Sekine, J.
PY - 2014/4/1
Y1 - 2014/4/1
N2 - The objective of this study was to identify the mechanism by which mandibular condyle chondrocytes regulate the extracellular matrix. Primary rabbit condylar chondrocytes were isolated, cultured, and treated with transforming growth factor beta 1 (TGF-β1). Cells were then assayed for the following: urokinase-type plasminogen activator (uPA) and its inhibitor (PAI-1), collagen types I and II, β1 integrin expression, and proliferative activity. TGF-β1 induced synthesis of collagen type II, αVβ1 integrin, and PAI-1. TGF-β1 induced the growth of chondrocytes and suppressed the synthesis of uPA. Chondrocyte regulation of the extracellular matrix is mediated by TGF-β1. Synthesis of collagen type II, αVβ1 integrin, and PAI-1 is induced, while uPA is suppressed. Also, TGF-β1 induces cellular growth.
AB - The objective of this study was to identify the mechanism by which mandibular condyle chondrocytes regulate the extracellular matrix. Primary rabbit condylar chondrocytes were isolated, cultured, and treated with transforming growth factor beta 1 (TGF-β1). Cells were then assayed for the following: urokinase-type plasminogen activator (uPA) and its inhibitor (PAI-1), collagen types I and II, β1 integrin expression, and proliferative activity. TGF-β1 induced synthesis of collagen type II, αVβ1 integrin, and PAI-1. TGF-β1 induced the growth of chondrocytes and suppressed the synthesis of uPA. Chondrocyte regulation of the extracellular matrix is mediated by TGF-β1. Synthesis of collagen type II, αVβ1 integrin, and PAI-1 is induced, while uPA is suppressed. Also, TGF-β1 induces cellular growth.
UR - http://www.scopus.com/inward/record.url?scp=84896729627&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84896729627&partnerID=8YFLogxK
U2 - 10.1016/j.ijom.2013.10.010
DO - 10.1016/j.ijom.2013.10.010
M3 - Article
C2 - 24252651
AN - SCOPUS:84896729627
VL - 43
SP - 470
EP - 475
JO - International Journal of Oral and Maxillofacial Surgery
JF - International Journal of Oral and Maxillofacial Surgery
SN - 0901-5027
IS - 4
ER -