Effects of transforming growth factor beta 1 on the plasminogen activation system, collagen and integrin synthesis, and proliferation of rabbit mandibular condylar chondrocytes

H. Ishibashi, Y. Nariai, T. Kanno, M. Onimaru, J. Sekine

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The objective of this study was to identify the mechanism by which mandibular condyle chondrocytes regulate the extracellular matrix. Primary rabbit condylar chondrocytes were isolated, cultured, and treated with transforming growth factor beta 1 (TGF-β1). Cells were then assayed for the following: urokinase-type plasminogen activator (uPA) and its inhibitor (PAI-1), collagen types I and II, β1 integrin expression, and proliferative activity. TGF-β1 induced synthesis of collagen type II, αVβ1 integrin, and PAI-1. TGF-β1 induced the growth of chondrocytes and suppressed the synthesis of uPA. Chondrocyte regulation of the extracellular matrix is mediated by TGF-β1. Synthesis of collagen type II, αVβ1 integrin, and PAI-1 is induced, while uPA is suppressed. Also, TGF-β1 induces cellular growth.

Original languageEnglish
Pages (from-to)470-475
Number of pages6
JournalInternational Journal of Oral and Maxillofacial Surgery
Volume43
Issue number4
DOIs
Publication statusPublished - Apr 1 2014

Fingerprint

Plasminogen
Chondrocytes
Integrins
Transforming Growth Factor beta
Collagen
Collagen Type II
Rabbits
Plasminogen Activator Inhibitor 1
Urokinase-Type Plasminogen Activator
Extracellular Matrix
Mandibular Condyle
Plasminogen Inactivators
Growth
Collagen Type I

All Science Journal Classification (ASJC) codes

  • Surgery
  • Oral Surgery
  • Otorhinolaryngology

Cite this

@article{95408b2e41fe4a90bbbb9863e32fcab1,
title = "Effects of transforming growth factor beta 1 on the plasminogen activation system, collagen and integrin synthesis, and proliferation of rabbit mandibular condylar chondrocytes",
abstract = "The objective of this study was to identify the mechanism by which mandibular condyle chondrocytes regulate the extracellular matrix. Primary rabbit condylar chondrocytes were isolated, cultured, and treated with transforming growth factor beta 1 (TGF-β1). Cells were then assayed for the following: urokinase-type plasminogen activator (uPA) and its inhibitor (PAI-1), collagen types I and II, β1 integrin expression, and proliferative activity. TGF-β1 induced synthesis of collagen type II, αVβ1 integrin, and PAI-1. TGF-β1 induced the growth of chondrocytes and suppressed the synthesis of uPA. Chondrocyte regulation of the extracellular matrix is mediated by TGF-β1. Synthesis of collagen type II, αVβ1 integrin, and PAI-1 is induced, while uPA is suppressed. Also, TGF-β1 induces cellular growth.",
author = "H. Ishibashi and Y. Nariai and T. Kanno and M. Onimaru and J. Sekine",
year = "2014",
month = "4",
day = "1",
doi = "10.1016/j.ijom.2013.10.010",
language = "English",
volume = "43",
pages = "470--475",
journal = "International Journal of Oral and Maxillofacial Surgery",
issn = "0901-5027",
publisher = "Churchill Livingstone",
number = "4",

}

TY - JOUR

T1 - Effects of transforming growth factor beta 1 on the plasminogen activation system, collagen and integrin synthesis, and proliferation of rabbit mandibular condylar chondrocytes

AU - Ishibashi, H.

AU - Nariai, Y.

AU - Kanno, T.

AU - Onimaru, M.

AU - Sekine, J.

PY - 2014/4/1

Y1 - 2014/4/1

N2 - The objective of this study was to identify the mechanism by which mandibular condyle chondrocytes regulate the extracellular matrix. Primary rabbit condylar chondrocytes were isolated, cultured, and treated with transforming growth factor beta 1 (TGF-β1). Cells were then assayed for the following: urokinase-type plasminogen activator (uPA) and its inhibitor (PAI-1), collagen types I and II, β1 integrin expression, and proliferative activity. TGF-β1 induced synthesis of collagen type II, αVβ1 integrin, and PAI-1. TGF-β1 induced the growth of chondrocytes and suppressed the synthesis of uPA. Chondrocyte regulation of the extracellular matrix is mediated by TGF-β1. Synthesis of collagen type II, αVβ1 integrin, and PAI-1 is induced, while uPA is suppressed. Also, TGF-β1 induces cellular growth.

AB - The objective of this study was to identify the mechanism by which mandibular condyle chondrocytes regulate the extracellular matrix. Primary rabbit condylar chondrocytes were isolated, cultured, and treated with transforming growth factor beta 1 (TGF-β1). Cells were then assayed for the following: urokinase-type plasminogen activator (uPA) and its inhibitor (PAI-1), collagen types I and II, β1 integrin expression, and proliferative activity. TGF-β1 induced synthesis of collagen type II, αVβ1 integrin, and PAI-1. TGF-β1 induced the growth of chondrocytes and suppressed the synthesis of uPA. Chondrocyte regulation of the extracellular matrix is mediated by TGF-β1. Synthesis of collagen type II, αVβ1 integrin, and PAI-1 is induced, while uPA is suppressed. Also, TGF-β1 induces cellular growth.

UR - http://www.scopus.com/inward/record.url?scp=84896729627&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84896729627&partnerID=8YFLogxK

U2 - 10.1016/j.ijom.2013.10.010

DO - 10.1016/j.ijom.2013.10.010

M3 - Article

C2 - 24252651

AN - SCOPUS:84896729627

VL - 43

SP - 470

EP - 475

JO - International Journal of Oral and Maxillofacial Surgery

JF - International Journal of Oral and Maxillofacial Surgery

SN - 0901-5027

IS - 4

ER -