TY - JOUR
T1 - Effects of tributyltin maternal and/or waterborne exposure on the embryonic development of the Manila clam, Ruditapes philippinarum
AU - Inoue, Suguru
AU - Oshima, Yuji
AU - Usuki, Hironori
AU - Hamaguchi, Masami
AU - Hanamura, Yukio
AU - Kai, Norihisa
AU - Shimasaki, Yohei
AU - Honjo, Tsuneo
N1 - Funding Information:
This study was partly supported by a grant from the Ministry of Education, Culture, Sports, Science and Technology, Japan (No. 14360112). We thank the staff of the National Research Institute of Fisheries and Environment of Inland Sea and also Mr. Jumpei Arakawa of the Aichi Fisheries Research Institute for their help with our experiment.
PY - 2006/5
Y1 - 2006/5
N2 - We examined the effect of tributyltin (TBT) on embryonic development of the Manila clam, Ruditapes philippinarum. In a maternal exposure test, 100 clams were exposed to TBT at measured concentrations of <0.01 (control), 0.061, 0.310, or 0.350 μg/l at 20-22 °C for 3 weeks, and the embryo developmental success (the ratio of normal D-larvae to all larvae) was measured. There was a significant negative correlation between embryo developmental success and TBT concentration in the female Manila clams (p < 0.001). These results indicated that TBT accumulated in the female clam decreased embryo developmental success. In a waterborne exposure test, fertilized eggs (4 h after fertilization) were exposed to TBT at measured concentrations of <0.01 (control), 0.062, 0.140, 0.320, or 0.640 μg/l for 23 h. Embryo developmental success was also significantly decreased in all TBT treatment groups compared with that in the control group. TBT accumulated in female adults and waterborne TBT clearly inhibit reproductive success of the clam.
AB - We examined the effect of tributyltin (TBT) on embryonic development of the Manila clam, Ruditapes philippinarum. In a maternal exposure test, 100 clams were exposed to TBT at measured concentrations of <0.01 (control), 0.061, 0.310, or 0.350 μg/l at 20-22 °C for 3 weeks, and the embryo developmental success (the ratio of normal D-larvae to all larvae) was measured. There was a significant negative correlation between embryo developmental success and TBT concentration in the female Manila clams (p < 0.001). These results indicated that TBT accumulated in the female clam decreased embryo developmental success. In a waterborne exposure test, fertilized eggs (4 h after fertilization) were exposed to TBT at measured concentrations of <0.01 (control), 0.062, 0.140, 0.320, or 0.640 μg/l for 23 h. Embryo developmental success was also significantly decreased in all TBT treatment groups compared with that in the control group. TBT accumulated in female adults and waterborne TBT clearly inhibit reproductive success of the clam.
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U2 - 10.1016/j.chemosphere.2005.07.047
DO - 10.1016/j.chemosphere.2005.07.047
M3 - Article
C2 - 16169053
AN - SCOPUS:33646049643
VL - 63
SP - 881
EP - 888
JO - Chemosphere
JF - Chemosphere
SN - 0045-6535
IS - 5
ER -
