Effects of type IV collagen on myogenic characteristics of IGF-I gene-engineered myoblasts

Akira Ito, Masahiro Yamamoto, Kazushi Ikeda, Masanori Sato, Kawabe Yoshinori, Masamichi Kamihira

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Skeletal muscle regeneration requires migration, proliferation and fusion of myoblasts to form multinucleated myotubes. In our previous study, we showed that insulin-like growth factor (IGF)-I gene delivery stimulates the proliferation and differentiation of mouse myoblast C2C12 cells and promotes the contractile force generated by tissue-engineered skeletal muscles. The aim of this study was to investigate the effects of the extracellular matrix on IGF-I gene-engineered C2C12 cells in vitro. Retroviral vectors for doxycycline (Dox)-inducible expression of the IGF-I gene were transduced into C2C12 cells. When cultured on a type IV collagen-coated surface, we observed significant increases in the migration speed and number of IGF-I gene-engineered C2C12 cells with Dox addition, designated as C2C12/IGF (+) cells. Co-culture of C2C12/IGF (+) cells and parental C2C12 cells, which had been cultured in differentiation medium for 3 days, greatly enhanced myotube formation. Moreover, type IV collagen supplementation promoted the fusion of C2C12/IGF (+) cells with differentiated C2C12 cells and increased the number of myotubes with striations. Myotubes formed by C2C12/IGF (+) cells cultured on type IV collagen showed a dynamic contractile activity in response to electrical pulse stimulation. These findings indicate that type IV collagen promotes skeletal muscle regeneration mediated by IGF-I-expressing myoblasts, which may have important clinical implications in the design of myoblast-based therapies.

Original languageEnglish
Pages (from-to)596-603
Number of pages8
JournalJournal of Bioscience and Bioengineering
Volume119
Issue number5
DOIs
Publication statusPublished - May 1 2015

Fingerprint

Collagen Type IV
Myoblasts
Insulin
Insulin-Like Growth Factor I
Collagen
Somatomedins
Genes
Skeletal Muscle Fibers
Muscle
Doxycycline
Fusion reactions
Skeletal Muscle
Regeneration
Intercellular Signaling Peptides and Proteins
Tissue
Coculture Techniques
Electric Stimulation
Extracellular Matrix
Cultured Cells
Cell Count

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Effects of type IV collagen on myogenic characteristics of IGF-I gene-engineered myoblasts. / Ito, Akira; Yamamoto, Masahiro; Ikeda, Kazushi; Sato, Masanori; Yoshinori, Kawabe; Kamihira, Masamichi.

In: Journal of Bioscience and Bioengineering, Vol. 119, No. 5, 01.05.2015, p. 596-603.

Research output: Contribution to journalArticle

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