Effects of U-37883A on intracellular Ca 2+-activated large-conductance K + channels in pig proximal urethral myocytes

Noriyoshi Teramoto, Manami Aishima, Hai Lei Zhu, Toshihisa Tomoda, Takakazu Yunoki, Fumi Takahashi, Alison F. Brading, Yushi Ito

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Kinetic studies of U-37883A (4-morpholinecarboximidine-N-1-adamantyl- N′-cyclohexyl-hydrochloride), a vascular ATP-sensitive K + channel (K ATP channel) blocker, were performed on pig urethral myocytes to investigate inhibitory effects on large-conductance intracellular Ca 2+-sensitive K + channels (i.e., BK Ca channels; 225 pS K + channels) by use of single-channel recordings (outside-out and inside-out configuration). BK Ca channels in pig urethral smooth muscles showed extracellular iberiotoxin (300 nM) sensitivity and voltage dependency. The α subunit of BK Ca channel proteins was detected in the membrane fraction by use of Western blot technique. Application of U-37883A (≥10 μM) reduced the activity of BK Ca channels in a concentration-dependent manner, not only by decreasing mean openlife time but also by prolonging the mean closed time. These results shows that U-37883A affects channels other than the vascular K ATP channel, and demonstrates how it inhibits the activities of BK Ca channels in urethral smooth muscles.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalEuropean Journal of Pharmacology
Volume506
Issue number1
DOIs
Publication statusPublished - Dec 3 2004

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Large-Conductance Calcium-Activated Potassium Channels
Muscle Cells
Swine
Adenosine Triphosphate
Smooth Muscle
Blood Vessels
Western Blotting
U 37883A
Membranes
Proteins

All Science Journal Classification (ASJC) codes

  • Pharmacology

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Effects of U-37883A on intracellular Ca 2+-activated large-conductance K + channels in pig proximal urethral myocytes. / Teramoto, Noriyoshi; Aishima, Manami; Zhu, Hai Lei; Tomoda, Toshihisa; Yunoki, Takakazu; Takahashi, Fumi; Brading, Alison F.; Ito, Yushi.

In: European Journal of Pharmacology, Vol. 506, No. 1, 03.12.2004, p. 1-7.

Research output: Contribution to journalArticle

Teramoto, N, Aishima, M, Zhu, HL, Tomoda, T, Yunoki, T, Takahashi, F, Brading, AF & Ito, Y 2004, 'Effects of U-37883A on intracellular Ca 2+-activated large-conductance K + channels in pig proximal urethral myocytes', European Journal of Pharmacology, vol. 506, no. 1, pp. 1-7. https://doi.org/10.1016/j.ejphar.2004.10.025
Teramoto, Noriyoshi ; Aishima, Manami ; Zhu, Hai Lei ; Tomoda, Toshihisa ; Yunoki, Takakazu ; Takahashi, Fumi ; Brading, Alison F. ; Ito, Yushi. / Effects of U-37883A on intracellular Ca 2+-activated large-conductance K + channels in pig proximal urethral myocytes. In: European Journal of Pharmacology. 2004 ; Vol. 506, No. 1. pp. 1-7.
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