Effects of UV Dose on Formation of Spontaneously Developing Pocks in Streptomyces azureus ATCC14921

Shuji Yamada, Hikaru Suenaga, Katsumi Doi, Sadazo Yoshino, Seiya Ogata

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Spontaneously developing pocks (S pocks) of Streptomyces azureus ATCC14921 were formed by the both functions of conjugative plasmid pSA1 and lysogenic phage SAt2. The formation was affected by the dose of UV irradiation. The mean pock diameter in cultures treated with UV light at 0, 7.1, 14.2 and 21.3×102 μW·erg/cm, respectively, were 1.3, 0.4, 2.2, and 0.5 mm. The dose affected conjugative plasmid pSA1 related to pock formation. There was UV damage of autonomous pSA1 replicon and UV induction of the chromosomal integrated sequence. Increases and decreases in the amount of autonomous pSA1 replicon corresponded to increases and decreases, respectively, in the diameter of the pocks. Both pSA1 and SAt2 syntheses were developed in the large pocks (1.3 and 2.2 mm), but only SAt2 synthesis was developed in the pinhole pocks (0.4 and 0.5 mm).

Original languageEnglish
Pages (from-to)797-802
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume67
Issue number4
DOIs
Publication statusPublished - Jan 1 2003

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Replicon
Streptomyces
Plasmids
Bacteriophages
Ultraviolet Rays
Ultraviolet radiation
Dosimetry
Irradiation

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Effects of UV Dose on Formation of Spontaneously Developing Pocks in Streptomyces azureus ATCC14921. / Yamada, Shuji; Suenaga, Hikaru; Doi, Katsumi; Yoshino, Sadazo; Ogata, Seiya.

In: Bioscience, Biotechnology and Biochemistry, Vol. 67, No. 4, 01.01.2003, p. 797-802.

Research output: Contribution to journalArticle

Yamada, Shuji ; Suenaga, Hikaru ; Doi, Katsumi ; Yoshino, Sadazo ; Ogata, Seiya. / Effects of UV Dose on Formation of Spontaneously Developing Pocks in Streptomyces azureus ATCC14921. In: Bioscience, Biotechnology and Biochemistry. 2003 ; Vol. 67, No. 4. pp. 797-802.
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