Effects of volatile and intravenous anesthetics on the uptake of GABA, glutamate and dopamine by their transporters heterologously expressed in COS cells and in rat brain synaptosomes

Mitsutaka Sugimura, Shigeo Kitayama, Katsuya Morita, Masahiro Irifune, Tohru Takarada, Michio Kawahara, Toshihiro Dohi

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)

Abstract

Although the neurotransmitter uptake system is considered a possible target for the presynaptic action of anesthetic agents, observations are inconsistent concerning effects on the transporter and their clinical relevance. The present study examined the effects of volatile and intravenous anesthetics on the uptake of GABA, glutamate and dopamine in COS cells heterologously expressing the transporters for these neurotransmitters and in the rat brain synaptosomes. Halothane and isoflurane, but not thiamylal or thiopental, significantly inhibited uptake by COS cell systems of GABA, dopamine and glutamic acid in a concentration-dependent manner within clinically relevant ranges for anesthesia induced by these agents. Similarly, in synaptosomes halothane and isoflurane but not thiopental significantly suppressed the uptake of GABA and glutamic acid, respectively. These results do not support the hypothesis that volatile and intravenous anesthetics exert their action via specific inhibition of GABA uptake to enhance inhibitory GABAergic neuronal activity. Rather, they suggest that presynaptic uptake systems for various neurotransmitters including GABA may be the molecular targets for volatile anesthetic agents.

Original languageEnglish
Pages (from-to)69-76
Number of pages8
JournalToxicology Letters
Volume123
Issue number1
DOIs
Publication statusPublished - Aug 6 2001
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Toxicology

Fingerprint Dive into the research topics of 'Effects of volatile and intravenous anesthetics on the uptake of GABA, glutamate and dopamine by their transporters heterologously expressed in COS cells and in rat brain synaptosomes'. Together they form a unique fingerprint.

Cite this