Efficient conversion of lactic acid to butanol with pH-stat continuous lactic acid and glucose feeding method by Clostridium saccharoperbutylacetonicum

Kenji Sonomoto, Mugihito Oshiro, Katsuhiro Hanada, Yukihiro Tashiro

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

In order to achieve high butanol production by Clostridium saccharoperbutylacetonicum N1-4, the effect of lactic acid on acetone-butanol-ethanol fermentation and several fed-batch cultures in which lactic acid is fed have been investigated. When a medium containing 20 g/l glucose was supplemented with 5 g/l of closely racemic lactic acid, both the concentration and yield of butanol increased; however, supplementation with more than 10 g/l lactic acid did not increase the butanol concentration. It was found that when fed a mixture of lactic acid and glucose, the final concentration of butanol produced by a fed-batch culture was greater than that produced by a batch culture. In addition, a pH-controlled fed-batch culture resulted in not only acceleration of lactic acid consumption but also a further increase in butanol production. Finally, we obtained 15.5 g/l butanol at a production rate of 1.76 g/l/h using a fed-batch culture with a pH-stat continuous lactic acid and glucose feeding method. To confirm whether lactic acid was converted to butanol by the N1-4 strain, we performed gas chromatography-mass spectroscopy (GC-MS) analysis of butanol produced by a batch culture during fermentation in a medium containing [1,2,3-13C3] lactic acid as the initial substrate. The results of the GC-MS analysis confirmed the bioconversion of lactic acid to butanol.

Original languageEnglish
Pages (from-to)1177-1185
Number of pages9
JournalApplied Microbiology and Biotechnology
Volume87
Issue number3
DOIs
Publication statusPublished - Jul 1 2010

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Feeding Methods
Butanols
Clostridium
Lactic Acid
Batch Cell Culture Techniques
Glucose
Gas Chromatography
Fermentation
Mass Spectrometry
Acetone
Ethanol

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Efficient conversion of lactic acid to butanol with pH-stat continuous lactic acid and glucose feeding method by Clostridium saccharoperbutylacetonicum. / Sonomoto, Kenji; Oshiro, Mugihito; Hanada, Katsuhiro; Tashiro, Yukihiro.

In: Applied Microbiology and Biotechnology, Vol. 87, No. 3, 01.07.2010, p. 1177-1185.

Research output: Contribution to journalArticle

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