Efficient delivery of siRNA using dendritic poly(l-lysine) for loss-of-function analysis

Yusuke Inoue, Ryosuke Kurihara, Akiko Tsuchida, Mino Hasegawa, Takeyuki Nagashima, Takeshi Mori, Takuro Niidome, Yoshiki Katayama, Osamu Okitsu

    Research output: Contribution to journalArticlepeer-review

    95 Citations (Scopus)

    Abstract

    RNA interference (RNAi) is a valuable tool for the validation of gene identification and functional genomics. Previously, it was reported that 6th generation dendritic poly(l-lysine) (KG6) transfected DNA into several cultivated cell lines with high efficiency and without any cytotoxic effects. In this study, the potential of KG6 to be an efficient siRNA carrier is investigated. KG6 showed effective knockdown of GAPDH with low cytotoxicity in combination with the weak-base amphiphilic peptide, Endo-Porter. In addition, the knockdown of PEPCK, which is the rate-limiting enzyme for gluconeogenesis, led to a reduction in glucose production in rat hepatoma H4IIEC3 cells. Knockdown of organic cation transporter 1 (OCT1), which is thought to be the gene that influences metformin action, was shown to successfully diminish the ability of metformin to inhibit gluconeogenesis in H4IIEC3 cells. In conclusion, using a combination of KG6 and Endo-Porter, a model system in which genes that influence metformin action can be identified was successfully constructed.

    Original languageEnglish
    Pages (from-to)59-66
    Number of pages8
    JournalJournal of Controlled Release
    Volume126
    Issue number1
    DOIs
    Publication statusPublished - Feb 18 2008

    All Science Journal Classification (ASJC) codes

    • Pharmaceutical Science

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