Efficient detection of protein-protein interactions on PMMA stereocomplex films

Hisao Matsuno, Yuya Nagasaka, Kimio Kurita, Takeshi Serizawa

Research output: Contribution to conferencePaperpeer-review

Abstract

Isotactic and syndiotactic poly(methyl methacrylate)s (PMMAs) form double-stranded helical stereocomplexes with a respective molar stoichiometry of 1:2 based on van der Waals interactions in organic solvents. Bioactivities of stereocomplex surfaces are significant for determining the potential for biomedical applications. We prepared PMMA stereocomplex films by a layer-by-layer assembly method, and the films were coated with protein A. Antibodies were deposited on the films, and then the binding of antigen was analyzed by QCM, AFM, and ATR-IR measurements. Specific binding amounts of HSA on Anti-HSA-coated stereocomplex films were greater than other surfaces and activity of the immobilized protein A on stereocomplex films was maintained for 10 days. These observations indicate that the surfaces of stereocomplex films were superior to conventional films for analysis of protein-protein interactions.

Original languageEnglish
Pages5153-5154
Number of pages2
Publication statusPublished - Dec 1 2005
Externally publishedYes
Event54th SPSJ Symposium on Macromolecules - Yamagata, Japan
Duration: Sep 20 2005Sep 22 2005

Other

Other54th SPSJ Symposium on Macromolecules
CountryJapan
CityYamagata
Period9/20/059/22/05

All Science Journal Classification (ASJC) codes

  • Engineering(all)

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