Efficient extracellular expression of a foreign protein in smptomyos using secretory protease inhibitor (SSI) gene fusions

Seiichi Taguchit, Izumi Kumagai, Jiro Nakayama, Akinori Suzuki, Kin Ichiro Miura

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Using the gene for a secreted protease inhibitor, Streptomyces subtilisin inhibitor (SSI), we have established a secretory expression system for the products of foreign genes in Streptomyces. A sex pheromone peptide, cADl, of Enterococcus fae- calis was expressed and secreted as a stable complete fusion protein with SSI in amounts comparable to the native SSI in S. lividans 66. Recombinant cADl, isolated from the fusion protein by chemical digestion with BrCN, has the same amino acid composition and sequence, retention time on reverse phase HPLC, molecular mass, and biological activity as authentic cADl. This system should be useful for the efficient expression of other foreign gene products, and as a model for heterologous gene expression in Streptomyces.

Original languageEnglish
Pages (from-to)1063-1066
Number of pages4
JournalNature Biotechnology
Volume7
Issue number10
DOIs
Publication statusPublished - Jan 1 1989

Fingerprint

Gene Fusion
Streptomyces
Protease Inhibitors
Fusion reactions
Genes
Proteins
Sex Attractants
Subtilisin
Enterococcus faecalis
Molecular mass
Bioactivity
Gene expression
Peptides
Proteolysis
Amino acids
Amino Acid Sequence
High Pressure Liquid Chromatography
Gene Expression
Amino Acids
Chemical analysis

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Medicine
  • Biomedical Engineering

Cite this

Efficient extracellular expression of a foreign protein in smptomyos using secretory protease inhibitor (SSI) gene fusions. / Taguchit, Seiichi; Kumagai, Izumi; Nakayama, Jiro; Suzuki, Akinori; Miura, Kin Ichiro.

In: Nature Biotechnology, Vol. 7, No. 10, 01.01.1989, p. 1063-1066.

Research output: Contribution to journalArticle

Taguchit, Seiichi ; Kumagai, Izumi ; Nakayama, Jiro ; Suzuki, Akinori ; Miura, Kin Ichiro. / Efficient extracellular expression of a foreign protein in smptomyos using secretory protease inhibitor (SSI) gene fusions. In: Nature Biotechnology. 1989 ; Vol. 7, No. 10. pp. 1063-1066.
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