Efficient gene transfer into silkworm larval tissues by a combination of sonoporation and lipofection

Jae Man Lee, Masateru Takahashi, Hiroaki Mon, Katsumi Koga, Yutaka Kawaguchi, Takahiro Kusakabe

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Sonoporation (ultrasound treatment) provides a new and attractive nonviral way of in vivo gene transfer. To access the applicability of this method to the silkworm, Bombyx mori, we have compared the efficiencies of gene transfer by means of lipofection (using an appropriate agent, PDD111), sonoporation (ditto, FluoroGene™), and lipofection followed by sonoporation. By these methods, a luciferase expression plasmid was found to be markedly transferred into the haemocoel of newly ecdysed fifth instar silkworm larvae, and also into other tissues although with lower rates compared with the haemocoel. In terms of luciferase activity, the efficiencies of transgene by lipofection plus sonoporation were approximately 6 (hemocytes), 20 (silk glands), 8 (mid-gut), 38 (fat body), 10 (Malpighian tubules), 33 (ovaries), and 16 (testes) times as high as those by lipofection or sonoporation alone. These results demonstrated that the present method is useful to introduce the exogenous DNA into insect organs in vivo.

Original languageEnglish
Pages (from-to)976-979
Number of pages4
JournalCell Biology International
Volume29
Issue number11
DOIs
Publication statusPublished - Nov 1 2005

Fingerprint

Bombyx
Luciferases
Malpighian Tubules
Genes
Hemocytes
Fat Body
Silk
Transgenes
Larva
Insects
Testis
Ovary
Plasmids
DNA

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Efficient gene transfer into silkworm larval tissues by a combination of sonoporation and lipofection. / Lee, Jae Man; Takahashi, Masateru; Mon, Hiroaki; Koga, Katsumi; Kawaguchi, Yutaka; Kusakabe, Takahiro.

In: Cell Biology International, Vol. 29, No. 11, 01.11.2005, p. 976-979.

Research output: Contribution to journalArticle

Lee, Jae Man ; Takahashi, Masateru ; Mon, Hiroaki ; Koga, Katsumi ; Kawaguchi, Yutaka ; Kusakabe, Takahiro. / Efficient gene transfer into silkworm larval tissues by a combination of sonoporation and lipofection. In: Cell Biology International. 2005 ; Vol. 29, No. 11. pp. 976-979.
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