Efficient gene transfer to airway epithelium using recombinant Sendai virus

Yoshikazu Yonemitsu, Christopher Kitson, Stefano Ferrari, Raymond Farley, Uta Griesenbach, Diane Judd, Rachel Steel, Philippe Scheid, Jie Zhu, Peter K. Jeffery, Atsushi Kato, Mohammad K. Hasan, Yoshiyuki Nagai, Ichiro Masaki, Masayuki Fukumura, Mamoru Hasegawa, Duncan M. Geddes, Eric W.F.W. Alton

Research output: Contribution to journalArticlepeer-review

206 Citations (Scopus)

Abstract

Clinical studies of gene therapy for cystic fibrosis (CF) suggest that the key problem is the efficiency of gene transfer to the airway epithelium. The availability of relevant vector receptors, the transient contact time between vector and epithelium, and the barrier function of airway mucus contribute significantly to this problem. We have recently developed recombinant Sendai virus (SeV) as a new gene transfer agent. Here we show that SeV produces efficient transfection throughout the respiratory tract of both mice and ferrets in vivo, as well as in freshly obtained human nasal epithelial cells in vitro. Gene transfer efficiency was several log orders greater than with cationic liposomes or adenovirus. Even very brief contact time was sufficient to produce this effect, and levels of expression were not significantly reduced by airway mucus. Our investigations suggest that SeV may provide a useful new vector for airway gene transfer.

Original languageEnglish
Pages (from-to)970-973
Number of pages4
JournalNature Biotechnology
Volume18
Issue number9
DOIs
Publication statusPublished - 2000
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Medicine
  • Biomedical Engineering

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