Efficient nonviral gene transfer mediated by polyethylenimine in an insect cell line

Takuji Maeda, Takahiro Kusakabe, Man Lee, Yoshitaka Miyagawa, Katsumi Koga, Yutaka Kawaguchi

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

An enhanced gene delivery system, monitored by the expression of a luciferase gene, was devised for an insect cell line BmN4, by altering the ratios of several liposome components. First, three kinds of commercially available polyethylenimine (PEI) reagents with different sizes were tested; one (PEI reagent with an average molecular weight of 1,800) gave a much better transfection efficiency than others. Using this particular reagent (PEI-1800), we newly formulated a liposome composed of three polycationic lipids: dioleoylphosphatidylchorine (DOTAP), dioleoylphosphatidylethanolamine (DOPE) and PEI-1800 in a ratio of 1:1:1. The current liposome gave a 2.5-fold efficiency compared to a commercially available liposome (CellFECTIN), a 2 : 3 mixture of TM-TPS (tetramethyl-tetrapalmitylspermine) and DOPE. The effects of divalent cations on the transfection with the newly devised liposome were investigated in detail. It is concluded that a mixture containing the PEI-1800 reagent provides a low cost and stable transfection procedure for the cultured silkworm cell line.

Original languageEnglish
Pages (from-to)21-26
Number of pages6
JournalJournal of Insect Biotechnology and Sericology
Volume74
Issue number1
Publication statusPublished - Feb 2005

Fingerprint

Gene transfer
Polyethyleneimine
Liposomes
transfection
gene transfer
Insects
Cells
cell lines
Cell Line
insects
Transfection
Genes
luciferase
silkworms
cations
Gene Transfer Techniques
Bombyx
molecular weight
Divalent Cations
Luciferases

All Science Journal Classification (ASJC) codes

  • Insect Science
  • Business, Management and Accounting(all)
  • Industrial and Manufacturing Engineering
  • Agricultural and Biological Sciences(all)
  • Polymers and Plastics

Cite this

Efficient nonviral gene transfer mediated by polyethylenimine in an insect cell line. / Maeda, Takuji; Kusakabe, Takahiro; Lee, Man; Miyagawa, Yoshitaka; Koga, Katsumi; Kawaguchi, Yutaka.

In: Journal of Insect Biotechnology and Sericology, Vol. 74, No. 1, 02.2005, p. 21-26.

Research output: Contribution to journalArticle

Maeda, Takuji ; Kusakabe, Takahiro ; Lee, Man ; Miyagawa, Yoshitaka ; Koga, Katsumi ; Kawaguchi, Yutaka. / Efficient nonviral gene transfer mediated by polyethylenimine in an insect cell line. In: Journal of Insect Biotechnology and Sericology. 2005 ; Vol. 74, No. 1. pp. 21-26.
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