Efficient production of recombinant human erythropoietin by replenishment of microcarriers in the hollow fiber culture cassette

Yuichi Inoue, Nobuhisa Arita, Kiichiro Teruya, Yoshinori Katakura, Sanetaka Shirahata

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Human erythropoietin (EPO)-producing recombinant BHK cells were cultured in culture medium containing microcarriers, and then microcarriers attached with cells were replenished in the hollow fiber culture cassette. By culture for 14 days, it was possible to produce 450 μg of the recombinant EPO, which corresponded to over two-fold of the recombinant EPO production by control hollow fiber culture without microcarriers.

Original languageEnglish
Pages (from-to)1624-1626
Number of pages3
JournalBioscience, Biotechnology and Biochemistry
Volume63
Issue number9
DOIs
Publication statusPublished - Jan 1 1999

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Erythropoietin
Fibers
Culture Media
Cultured Cells

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

Efficient production of recombinant human erythropoietin by replenishment of microcarriers in the hollow fiber culture cassette. / Inoue, Yuichi; Arita, Nobuhisa; Teruya, Kiichiro; Katakura, Yoshinori; Shirahata, Sanetaka.

In: Bioscience, Biotechnology and Biochemistry, Vol. 63, No. 9, 01.01.1999, p. 1624-1626.

Research output: Contribution to journalArticle

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