Efficient regeneration of Eucommia ulmoides from hypocotyl explant

R. Chen; S. Namimatsu; Y. Nakadozono; T. Bamba; Y. Nakazawa; K. Gyokusen

doi:10.1007/s10535-008-0137-x

Efficient regeneration of Eucommia ulmoides from hypocotyl explant

R. Chen, S. Namimatsu, Y. Nakadozono, T. Bamba, Y. Nakazawa, K. Gyokusen

Forest Sciences

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

A rapid and efficient method for the regeneration of Eucommia ulmoides Oliver has been developed. The ability of hypocotyl segments to produce adventitious buds varied depending upon their position, seedling age and culture medium. The most adventitious buds were induced from the hypocotyl segments near to the root of 2-week-old seedlings on the Murashige and Skoog (MS) basal medium supplemented with 10 μM benzylaminopurine (BAP). However, the MS medium with 6 μM BAP and 6 μM gibberellic acid (GA₃) was most suitable for shoot multiplication and further growth. Shoots longer than 2 cm cultured on the half-strength MS medium supplemented with 0.5 μM naphthaleneacetic acid (NAA) produced a maximum number of roots per shoot. Regenerated plantlets could be successfully acclimatized.

Original language	English
Pages (from-to)	713-717
Number of pages	5
Journal	Biologia Plantarum
Volume	52
Issue number	4
DOIs	https://doi.org/10.1007/s10535-008-0137-x
Publication status	Published - Dec 2008

All Science Journal Classification (ASJC) codes

Plant Science
Horticulture

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title = "Efficient regeneration of Eucommia ulmoides from hypocotyl explant",

abstract = "A rapid and efficient method for the regeneration of Eucommia ulmoides Oliver has been developed. The ability of hypocotyl segments to produce adventitious buds varied depending upon their position, seedling age and culture medium. The most adventitious buds were induced from the hypocotyl segments near to the root of 2-week-old seedlings on the Murashige and Skoog (MS) basal medium supplemented with 10 μM benzylaminopurine (BAP). However, the MS medium with 6 μM BAP and 6 μM gibberellic acid (GA3) was most suitable for shoot multiplication and further growth. Shoots longer than 2 cm cultured on the half-strength MS medium supplemented with 0.5 μM naphthaleneacetic acid (NAA) produced a maximum number of roots per shoot. Regenerated plantlets could be successfully acclimatized.",

author = "R. Chen and S. Namimatsu and Y. Nakadozono and T. Bamba and Y. Nakazawa and K. Gyokusen",

note = "Funding Information: Received 27 June 2006, accepted 16 May 2007. Abbreviations: BAP - 6-benzylaminopurine; GA3 - gibberellic acid; IAA - 3-indoleacetic acid; IBA - 3-indolebutyric acid; MS medium -Murashige and Skoog medium (1962); NAA - naphthaleneacetic acid. Acknowledgements: This work was performed as one of the technology development projects of the “Program for the Creation Recycling-Based Industrial Systems Using Biological Functions, Development of Transgenic Plants for Production of Industrial Materials” supported by NEDO (New Energy and Industrial Technology Development Organization), Japan. * Corresponding author; fax: (+81) 92 642 2873, e-mail: chenren@agr.kyushu-u.ac.jp",

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AU - Nakazawa, Y.

AU - Gyokusen, K.

N1 - Funding Information: Received 27 June 2006, accepted 16 May 2007. Abbreviations: BAP - 6-benzylaminopurine; GA3 - gibberellic acid; IAA - 3-indoleacetic acid; IBA - 3-indolebutyric acid; MS medium -Murashige and Skoog medium (1962); NAA - naphthaleneacetic acid. Acknowledgements: This work was performed as one of the technology development projects of the “Program for the Creation Recycling-Based Industrial Systems Using Biological Functions, Development of Transgenic Plants for Production of Industrial Materials” supported by NEDO (New Energy and Industrial Technology Development Organization), Japan. * Corresponding author; fax: (+81) 92 642 2873, e-mail: chenren@agr.kyushu-u.ac.jp

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Efficient regeneration of Eucommia ulmoides from hypocotyl explant

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