EGR2 induces apoptosis in various cancer cell lines by direct transactivation of BNIP3L and BAK

Motoko Unoki, Yusuke Nakamura

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

EGR2 plays a key role in the PTEN-induced apoptotic pathway. Using adenovirus-mediated gene transfer to 39 cancer cell lines, we found that EGR2 could induce apoptosis in a large proportion of these lines by altering the permeability of mitochondrial membranes, releasing cytochrome c and activating caspase-3, -8, and -9. Analysis by cDNA microarray and subsequent functional studies revealed that EGR2 directly transactivates expression of BNIP3L and BAK. Our results helped to clarify the molecular mechanism of the apoptotic pathway induced by PTEN-EGR2, and suggested that EGR2 may be an excellent target molecule for gene therapy to treat a variety of cancers.

Original languageEnglish
Pages (from-to)2172-2185
Number of pages14
JournalOncogene
Volume22
Issue number14
DOIs
Publication statusPublished - Apr 10 2003
Externally publishedYes

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Transcriptional Activation
Apoptosis
Cell Line
Caspase 8
Mitochondrial Membranes
Cytochromes c
Oligonucleotide Array Sequence Analysis
Adenoviridae
Caspase 3
Genetic Therapy
Permeability
Neoplasms
Genes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

EGR2 induces apoptosis in various cancer cell lines by direct transactivation of BNIP3L and BAK. / Unoki, Motoko; Nakamura, Yusuke.

In: Oncogene, Vol. 22, No. 14, 10.04.2003, p. 2172-2185.

Research output: Contribution to journalArticle

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