Eicosapentaenoic acid modifies cytokine activity and inhibits cell proliferation in an oesophageal cancer cell line

Hisako Kubota, Hideo Matsumoto, Masaharu Higashida, Haruaki Murakami, Hiroshi Nakashima, Yasuo Oka, Hideo Okumura, Masahiro Yamamura, Masahumi Nakamura, Toshihiro Hirai

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12 Citations (Scopus)

Abstract

Background/Aim: The present study investigated the effect of eicosapentaenoic acid (EPA) on nuclear factor-kappa B (NF-κB) activation, inflammatory interleukin-6 (IL-6) production, and cell proliferation in a human oesophageal carcinoma cell line (TE-1). Materials and Methods: Lipopolysaccharide (LPS)-induced IL-6 production in TE-1 cells in the presence or absence of EPA was determined using enzyme-linked immunosorbent assay (ELISA). The proliferation of TE-1 cells was determined by the WST-1 assay. TE-1 cells were stained with Hoechst 33342 and propidium iodide to observe apoptosis. Immunohistochemical staining of NF-κB in TE-1 cells was performed. Results: LPS increased IL-6 production in TE-1 cells, and EPA treatment prevented this effect. EPA treatment inhibited NF-κB activation and induced apoptosis of TE-1 cells. Conclusion: EPA inhibits NF-κB activation and IL-6 production in oesophageal cancer cells, their inducing apoptosis. These effects of EPA may be of benefit in improving the outcome of cancer surgery.

Original languageEnglish
Pages (from-to)4319-4324
Number of pages6
JournalAnticancer research
Volume33
Issue number10
Publication statusPublished - Oct 1 2013
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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    Kubota, H., Matsumoto, H., Higashida, M., Murakami, H., Nakashima, H., Oka, Y., Okumura, H., Yamamura, M., Nakamura, M., & Hirai, T. (2013). Eicosapentaenoic acid modifies cytokine activity and inhibits cell proliferation in an oesophageal cancer cell line. Anticancer research, 33(10), 4319-4324.