Electric knife for cell surgery: Local ablation by micro-plasma discharge

Y. Yamanishi, H. Kuriki, S. Sakuma, K. Onda, T. Kawahara, F. Arai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

We have firstly succeeded in enucleation of oocyte by using micro-electric knife. The discharged output power and conductive area were controlled to adapt the cellular-order ablation under water and under atmospheric pressure environment. The local area ablation was obtained by the glass shell insulation around the copper wire with silver paste, and which prevent the bubble generation and protein contamination when it was discharged under the water. The width of the ablation region was successfully reduced to more than a half by using the glass shell insulation compare to the conventional electrode. Moreover, the nucleus dyed by Hoechst was also removed successfully with limited damage region which is the difficult task for manual operation with glass capillary. This low cost micro-electric knife has a possibility to extend to fabricate any objective material under various environments and contribute to a new top-down fabrication method in the micro-nano bioengineering field.

Original languageEnglish
Title of host publication2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
Pages945-948
Number of pages4
DOIs
Publication statusPublished - May 7 2012
Externally publishedYes
Event2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012 - Paris, France
Duration: Jan 29 2012Feb 2 2012

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)1084-6999

Other

Other2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
CountryFrance
CityParis
Period1/29/122/2/12

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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  • Cite this

    Yamanishi, Y., Kuriki, H., Sakuma, S., Onda, K., Kawahara, T., & Arai, F. (2012). Electric knife for cell surgery: Local ablation by micro-plasma discharge. In 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012 (pp. 945-948). [6170342] (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2012.6170342