Electrical detection of norovirus capsid using dielectrophoretic impedance measurement method

Michihiko Nakano, Takafumi Hisajima, Lina Mao, Junya Suehiro

Research output: Chapter in Book/Report/Conference proceedingConference contribution

5 Citations (Scopus)

Abstract

In this work, we demonstrated an electrical detection technique of norovirus capsid in liquid by using dielectrophoretic impedance measurement (DEPIM). DEPIM is a method composing of capturing target particles by dielectrophoresis (DEP) in the gap of microelectrodes and measuring the impedance change of the microelectrodes caused by the captured particles. DEPIM has been successfully applied to bacteria detection by the present authors. This is the first report to extend scope of DEPIM inspection from bacteria to norovirus. Recombinant norovirus capsid was employed as target particles in experiments due to safety concerns. First, we showed virus capturing by DEP using fluorescent labeled norovirus capsid. Then, DEPIM of the capsid was carried out. As the result, DEPIM detected 2.5 ng/ml of the norovirus capsid in 300 sec. This implies that DEPIM has comparable sensitivity but shorter detection time than immunochromatography.

Original languageEnglish
Title of host publicationIEEE SENSORS 2012 - Proceedings
DOIs
Publication statusPublished - Dec 1 2012
Event11th IEEE SENSORS 2012 Conference - Taipei, Taiwan, Province of China
Duration: Oct 28 2012Oct 31 2012

Publication series

NameProceedings of IEEE Sensors

Other

Other11th IEEE SENSORS 2012 Conference
CountryTaiwan, Province of China
CityTaipei
Period10/28/1210/31/12

All Science Journal Classification (ASJC) codes

  • Electrical and Electronic Engineering

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  • Cite this

    Nakano, M., Hisajima, T., Mao, L., & Suehiro, J. (2012). Electrical detection of norovirus capsid using dielectrophoretic impedance measurement method. In IEEE SENSORS 2012 - Proceedings [6411163] (Proceedings of IEEE Sensors). https://doi.org/10.1109/ICSENS.2012.6411163