Abstract
We have succeeded in protein crystallization and processing by electrically-induced bubble knife. The dispensed directional mono-dispersed micro-bubbles whose air-liquid interface tends to combine the ambient molecules due to by their stiction force on the surface of micro-bubbles. Electrically-induced bubble knife enables to generate mono-dispersed micro-bubbles by the electrolysis and local heat, and their generation speed was 30.8 kHz. These characteristics enable to agglomerate the protein molecule which brings to successful protein crystallization in the solution with low concentration. The rate of protein crystal-nucleation by electrically-induced mono-dispersed micro-bubble was much higher than that of control condition. Furthermore, it was confirmed that cavitation induced by electrically-induced bubble knife enabled to curve protein crystal. It seemed that it has potential to contribute to more refined analysis.
| Original language | English |
|---|---|
| Title of host publication | 2013 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2013 |
| Publisher | IEEE Computer Society |
| ISBN (Print) | 9781479915293 |
| DOIs | |
| Publication status | Published - 2013 |
| Externally published | Yes |
| Event | 2013 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2013 - Nagoya, Japan Duration: Nov 10 2013 → Nov 13 2013 |
Other
| Other | 2013 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2013 |
|---|---|
| Country | Japan |
| City | Nagoya |
| Period | 11/10/13 → 11/13/13 |
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All Science Journal Classification (ASJC) codes
- Electrical and Electronic Engineering
Cite this
Electrically-induced bubble knife for protein crystallization and processing. / Kuriki, Hiroki; Takasawa, Sou; Sakuma, Shinya; Kurisu, Genji; Arai, Fumihito; Yamanishi, Yoko.
2013 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2013. IEEE Computer Society, 2013. 6710474.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Electrically-induced bubble knife for protein crystallization and processing
AU - Kuriki, Hiroki
AU - Takasawa, Sou
AU - Sakuma, Shinya
AU - Kurisu, Genji
AU - Arai, Fumihito
AU - Yamanishi, Yoko
PY - 2013
Y1 - 2013
N2 - We have succeeded in protein crystallization and processing by electrically-induced bubble knife. The dispensed directional mono-dispersed micro-bubbles whose air-liquid interface tends to combine the ambient molecules due to by their stiction force on the surface of micro-bubbles. Electrically-induced bubble knife enables to generate mono-dispersed micro-bubbles by the electrolysis and local heat, and their generation speed was 30.8 kHz. These characteristics enable to agglomerate the protein molecule which brings to successful protein crystallization in the solution with low concentration. The rate of protein crystal-nucleation by electrically-induced mono-dispersed micro-bubble was much higher than that of control condition. Furthermore, it was confirmed that cavitation induced by electrically-induced bubble knife enabled to curve protein crystal. It seemed that it has potential to contribute to more refined analysis.
AB - We have succeeded in protein crystallization and processing by electrically-induced bubble knife. The dispensed directional mono-dispersed micro-bubbles whose air-liquid interface tends to combine the ambient molecules due to by their stiction force on the surface of micro-bubbles. Electrically-induced bubble knife enables to generate mono-dispersed micro-bubbles by the electrolysis and local heat, and their generation speed was 30.8 kHz. These characteristics enable to agglomerate the protein molecule which brings to successful protein crystallization in the solution with low concentration. The rate of protein crystal-nucleation by electrically-induced mono-dispersed micro-bubble was much higher than that of control condition. Furthermore, it was confirmed that cavitation induced by electrically-induced bubble knife enabled to curve protein crystal. It seemed that it has potential to contribute to more refined analysis.
UR - http://www.scopus.com/inward/record.url?scp=84893797876&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84893797876&partnerID=8YFLogxK
U2 - 10.1109/MHS.2013.6710474
DO - 10.1109/MHS.2013.6710474
M3 - Conference contribution
AN - SCOPUS:84893797876
SN - 9781479915293
BT - 2013 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2013
PB - IEEE Computer Society
ER -