Electrically-induced bubble knife for protein crystallization and processing

Hiroki Kuriki; Sou Takasawa; Shinya Sakuma; Genji Kurisu; Fumihito Arai; Yoko Yamanishi

doi:10.1109/MHS.2013.6710474

Electrically-induced bubble knife for protein crystallization and processing

Hiroki Kuriki, Sou Takasawa, Shinya Sakuma, Genji Kurisu, Fumihito Arai, Yoko Yamanishi

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We have succeeded in protein crystallization and processing by electrically-induced bubble knife. The dispensed directional mono-dispersed micro-bubbles whose air-liquid interface tends to combine the ambient molecules due to by their stiction force on the surface of micro-bubbles. Electrically-induced bubble knife enables to generate mono-dispersed micro-bubbles by the electrolysis and local heat, and their generation speed was 30.8 kHz. These characteristics enable to agglomerate the protein molecule which brings to successful protein crystallization in the solution with low concentration. The rate of protein crystal-nucleation by electrically-induced mono-dispersed micro-bubble was much higher than that of control condition. Furthermore, it was confirmed that cavitation induced by electrically-induced bubble knife enabled to curve protein crystal. It seemed that it has potential to contribute to more refined analysis.

Original language	English
Title of host publication	2013 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2013
Publisher	IEEE Computer Society
ISBN (Print)	9781479915293
DOIs	https://doi.org/10.1109/MHS.2013.6710474
Publication status	Published - 2013
Externally published	Yes
Event	2013 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2013 - Nagoya, Japan Duration: Nov 10 2013 → Nov 13 2013

Other

Other	2013 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2013
Country	Japan
City	Nagoya
Period	11/10/13 → 11/13/13

All Science Journal Classification (ASJC) codes

Electrical and Electronic Engineering

Access to Document

10.1109/MHS.2013.6710474

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Electrically-induced bubble knife for protein crystallization and processing. / Kuriki, Hiroki; Takasawa, Sou; Sakuma, Shinya; Kurisu, Genji; Arai, Fumihito; Yamanishi, Yoko.

2013 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2013. IEEE Computer Society, 2013. 6710474.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Kuriki, H, Takasawa, S, Sakuma, S, Kurisu, G, Arai, F & Yamanishi, Y 2013, Electrically-induced bubble knife for protein crystallization and processing. in 2013 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2013., 6710474, IEEE Computer Society, 2013 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2013, Nagoya, Japan, 11/10/13. https://doi.org/10.1109/MHS.2013.6710474

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abstract = "We have succeeded in protein crystallization and processing by electrically-induced bubble knife. The dispensed directional mono-dispersed micro-bubbles whose air-liquid interface tends to combine the ambient molecules due to by their stiction force on the surface of micro-bubbles. Electrically-induced bubble knife enables to generate mono-dispersed micro-bubbles by the electrolysis and local heat, and their generation speed was 30.8 kHz. These characteristics enable to agglomerate the protein molecule which brings to successful protein crystallization in the solution with low concentration. The rate of protein crystal-nucleation by electrically-induced mono-dispersed micro-bubble was much higher than that of control condition. Furthermore, it was confirmed that cavitation induced by electrically-induced bubble knife enabled to curve protein crystal. It seemed that it has potential to contribute to more refined analysis.",

author = "Hiroki Kuriki and Sou Takasawa and Shinya Sakuma and Genji Kurisu and Fumihito Arai and Yoko Yamanishi",

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AU - Kuriki, Hiroki

AU - Takasawa, Sou

AU - Sakuma, Shinya

AU - Kurisu, Genji

AU - Arai, Fumihito

AU - Yamanishi, Yoko

PY - 2013

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AB - We have succeeded in protein crystallization and processing by electrically-induced bubble knife. The dispensed directional mono-dispersed micro-bubbles whose air-liquid interface tends to combine the ambient molecules due to by their stiction force on the surface of micro-bubbles. Electrically-induced bubble knife enables to generate mono-dispersed micro-bubbles by the electrolysis and local heat, and their generation speed was 30.8 kHz. These characteristics enable to agglomerate the protein molecule which brings to successful protein crystallization in the solution with low concentration. The rate of protein crystal-nucleation by electrically-induced mono-dispersed micro-bubble was much higher than that of control condition. Furthermore, it was confirmed that cavitation induced by electrically-induced bubble knife enabled to curve protein crystal. It seemed that it has potential to contribute to more refined analysis.

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