Electro protoplast fusion between Solanum sisymbriifolium and other Solanum species

Naoki Oda, Shiro Isshiki, Takeshi Sadohara, Yukio Ozaki, Hiroshi Okubo

Research output: Contribution to journalArticle

Abstract

Symmetric or asymmetric protoplast fusions between Solanum sisymbriifolium and other Solanum species (S. integrifolium and S. toxicarium) were performed to obtain the perfectly soil-borne disease resistant rootstocks for eggplant cultivation. Somatic hybrid was not obtained from symmetric fusion in selective media, where one parent's protoplasts formed colonies and the other did not. Combining of iodoacetamide-induced inactivation of S. integrifolium and no cell division ability of S. sisymbriifolium on a selective medium in asymmetric fusion, the hybridity of two calli was elucidated by analyzing IDH, PGM and SKDH isozymes. Plant regeneration from the somatic hybrid calli was also successful, but the regenerated plants did not show the hybridity. The result may be caused by the elimination of S. integrifolium chromosomes during the process of shoot regeneration from calli.

Original languageEnglish
Pages (from-to)67-72
Number of pages6
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume51
Issue number1
Publication statusPublished - Feb 1 2006

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

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