Electrochemical assay for saccharide-protein interactions using glycopolymer-modified gold nanoparticles

Jin Ishii, Miyuki Chikae, Masayuki Toyoshima, Yoshiaki Ukita, Yoshiko Miura, Yuzuru Takamura

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

A novel recognition element, made of electroactive glycopolymer-modified gold nanoparticles (GM-GNPs), was synthesized and used in an electrochemical assay of saccharide-protein interactions for the detection of concanavalin A (Con A). The electroactivity of the mannose-based GM-GNPs was investigated, and an electrochemical Con A assay system with a disposable screen printed carbon strips was proposed. A complex containing GM-GNPs was formed by a recognition reaction between the mannose-based glycopolymer and Con A. The complex was detected by the pre-oxidation of the GNPs and following reduction scans using differential pulse voltammetry (DPV). Under optimal conditions, a linear relationship between the DPV peak current intensity and Con A concentration was shown within an analytical range of 10-10,000 ng/mL. Our proposed new electric approach provides an interesting tool for the analysis of saccharide-protein interactions.

Original languageEnglish
Pages (from-to)830-833
Number of pages4
JournalElectrochemistry Communications
Volume13
Issue number8
DOIs
Publication statusPublished - Aug 1 2011

All Science Journal Classification (ASJC) codes

  • Electrochemistry

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