Electrochemical control of bioluminescence by blocking the adsorption of the bacterial luciferase using a mercaptobipyridine self-assembled monolayer

Shinya Yamasaki, Shuto Yamada, Hiroyuki Takemura, Kô Takehara

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An N-butyl-N'-(4-mercaptobutyl)-4,4'-bipyridinium (4BMBP) was modified on a gold electrode to improve the electrochemical control of the bacterial luciferase (BL) luminescence system. The 4BMBP-modified gold electrode (4BMBP/Au) was able to prevent the adsorption of BL on the electrode surface, and enhanced the electrochemical regeneration rate of the reduced flavin mononucleotide (FMNH 2 ), which is one of the substrates of the BL luminescence reaction. By using the 4BMBP/Au, the luminescence intensity increased by about 27% compared to that of a bare gold electrode (bare Au). Moreover, the modified electrode improved the time required for analysis because the modified layer prevented BL adsorption. Even without a refreshing procedure for each measurement, a constant luminescence intensity could be observed, and the analysis time was reduced to half (about 10 min) for one sample. The 4BMBP/Au is not only useful to control of the BL luminescence system, but also for electrochemical measurements in the presence of proteins.

Original languageEnglish
Pages (from-to)307-311
Number of pages5
Journalanalytical sciences
Issue number3
Publication statusPublished - Jan 1 2017


All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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