Electrochemical extraction of proteins by reverse micelle formation

Mariko Shinshi, Takayasu Sugihara, Toshiyuki Osakai, Masahiro Goto

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

The transfer of proteins by the anionic surfactant bis(2-ethylhexyl) sulfosuccinate (AOT) at a polarized 1,2-dichloroethane/water (DCE/W) interface was investigated by means of ion-transfer voltammetry. When the tetrapentylammonium salt of AOT was added to the DCE phase, the facilitated transfer of certain proteins, including cytochrome c (Cyt c), ribonuclease A, and protamine, could be controlled electrochemically, and a well-defined anodic wave for the transfer was obtained. At low pH values (e.g., pH 3.4), the anodic wave was usually well-separated from the wave for the formation of protein-free (i.e., unfilled) reverse micelles. The anodic wave for the protein transfer was analyzed by applying the theory for facilitated transfer of ions by charged ligands and then supplying information regarding the number of AOT anions reacting with one protein molecule and the total charge carried by the protein transfer. However, controlled-potential electrolyses performed for the transfer of Cyt c, which is red, revealed that the protein-AOT complexes were unstable in DCE and liable to aggregate at the interface when the pH of the W phase was 3.4. At pH 7.0, when formation of unfilled reverse micelles occurred simultaneously, the protein-AOT complexes appeared to be stabilized, probably via fusion with unfilled reverse micelles.

Original languageEnglish
Pages (from-to)5937-5944
Number of pages8
JournalLangmuir
Volume22
Issue number13
DOIs
Publication statusPublished - Jun 20 2006

Fingerprint

Micelles
micelles
proteins
Proteins
cytochromes
Cytochromes c
Ions
Pancreatic Ribonuclease
Protamines
Anionic surfactants
supplying
electrolysis
Voltammetry
Electrolysis
Anions
ions
Fusion reactions
Salts
fusion
surfactants

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

Cite this

Electrochemical extraction of proteins by reverse micelle formation. / Shinshi, Mariko; Sugihara, Takayasu; Osakai, Toshiyuki; Goto, Masahiro.

In: Langmuir, Vol. 22, No. 13, 20.06.2006, p. 5937-5944.

Research output: Contribution to journalArticle

Shinshi, M, Sugihara, T, Osakai, T & Goto, M 2006, 'Electrochemical extraction of proteins by reverse micelle formation', Langmuir, vol. 22, no. 13, pp. 5937-5944. https://doi.org/10.1021/la060858n
Shinshi, Mariko ; Sugihara, Takayasu ; Osakai, Toshiyuki ; Goto, Masahiro. / Electrochemical extraction of proteins by reverse micelle formation. In: Langmuir. 2006 ; Vol. 22, No. 13. pp. 5937-5944.
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