Electrochemical properties of myoglobin embedded in Langmuir-Blodgett and cast films of synthetic lipids

Alaa Eldin F. Nassar, Yoshitaka Narikiyo, Takamasa Sagara, Naotoshi Nakashima, James F. Rusling

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    81 Citations (Scopus)

    Abstract

    Electrochemical parameters, formal potentials (Eo′), electron-transfer rate constants (ko′), and diffusion coefficients (Dct) have been determined for myoglobin (Mb) embedded in the Langmuir-Blodgett (LB) and cast films of five synthetic lipids including two types of polymeric lipids bearing ammonium or poly(ethylene glycol) as a head group. The heterogeneous electron-transfer rate constants of MbFeIII/MbFeII in these films were ca. 102-103-fold larger than those for Mb in solution at an indium tin oxide electrode. Myoglobin-lipid LB films on basal-plane pyrolytic graphite (PG) electrodes gave 3-10-fold larger ko′ values compared with cast films of the same lipid. The Mb-lipid films showed gel to liquid-crystal phase transitions consistent with bilayer structures. The peak current of the square wave voltammograms for the cast Mb-lipid films showed breaks in the phase-transition temperature regions of the corresponding lipid films. Dependence of ko′ on lipid structure was not significant. A variety of synthetic lipids provided suitable microenvironments for the enhanced electron transfer of Mb. Immobilized Mb in the lipid films was stable for more than a month. The specific orientation of Mb in all Mb-lipid films was shown by linear dichroism. The orientation does not depend on the type of the lipids.

    Original languageEnglish
    Pages (from-to)1775-1782
    Number of pages8
    JournalJournal of the Chemical Society, Faraday Transactions
    Volume91
    Issue number12
    DOIs
    Publication statusPublished - Dec 1 1995

    All Science Journal Classification (ASJC) codes

    • Physical and Theoretical Chemistry

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