Electrochemistry of cast films of ferredoxin and lipid bilayers on electrodes

Alaa Eldin F. Nassar, James F. Rusling, Masato Tominaga, Junko Yanagimoto, Naotoshi Nakashima

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

Structural stability and electrochemistry of chlorella ferredoxin immobilized in a cast film of molecular bilayer membranes of a biological lipid, dimyristoylphosphatidylcholine (DMPC) on a quartz substrate or a pyrolytic graphite electrode were examined. A specular reflectance spectrum of the film showed that the [2Fe-2S] cluster in ferredoxin retains its original structure even in an air atmosphere. This indicates that the lipid bilayers provide suitable microenvironments for a stable immobilization of the protein. Cast films of ferredoxin + DMPC on a graphite electrode gave well-defined, nearly reversible redox waves with a formal potential of -0.59 V vs. Ag|AgCl, which is close to the reported value at promoter-modified electrodes, indicating that the electrochemical communication is from native ferredoxin.

Original languageEnglish
Pages (from-to)183-185
Number of pages3
JournalJournal of Electroanalytical Chemistry
Volume416
Issue number1-2
DOIs
Publication statusPublished - Nov 1 1996

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Chemical Engineering(all)
  • Electrochemistry

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