Electrochemistry of cast films of ferredoxin and lipid bilayers on electrodes

Alaa Eldin F. Nassar, James F. Rusling, Masato Tominaga, Junko Yanagimoto, Naotoshi Nakashima

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Structural stability and electrochemistry of chlorella ferredoxin immobilized in a cast film of molecular bilayer membranes of a biological lipid, dimyristoylphosphatidylcholine (DMPC) on a quartz substrate or a pyrolytic graphite electrode were examined. A specular reflectance spectrum of the film showed that the [2Fe-2S] cluster in ferredoxin retains its original structure even in an air atmosphere. This indicates that the lipid bilayers provide suitable microenvironments for a stable immobilization of the protein. Cast films of ferredoxin + DMPC on a graphite electrode gave well-defined, nearly reversible redox waves with a formal potential of -0.59 V vs. Ag|AgCl, which is close to the reported value at promoter-modified electrodes, indicating that the electrochemical communication is from native ferredoxin.

Original languageEnglish
Pages (from-to)183-185
Number of pages3
JournalJournal of Electroanalytical Chemistry
Volume416
Issue number1-2
DOIs
Publication statusPublished - Nov 1 1996

Fingerprint

Ferredoxins
Lipid bilayers
Electrochemistry
Graphite electrodes
Dimyristoylphosphatidylcholine
Electrodes
Lipids
Quartz
Proteins
Membranes
Communication
Substrates
Air

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Chemical Engineering(all)
  • Electrochemistry

Cite this

Nassar, A. E. F., Rusling, J. F., Tominaga, M., Yanagimoto, J., & Nakashima, N. (1996). Electrochemistry of cast films of ferredoxin and lipid bilayers on electrodes. Journal of Electroanalytical Chemistry, 416(1-2), 183-185. https://doi.org/10.1016/S0022-0728(96)01013-3

Electrochemistry of cast films of ferredoxin and lipid bilayers on electrodes. / Nassar, Alaa Eldin F.; Rusling, James F.; Tominaga, Masato; Yanagimoto, Junko; Nakashima, Naotoshi.

In: Journal of Electroanalytical Chemistry, Vol. 416, No. 1-2, 01.11.1996, p. 183-185.

Research output: Contribution to journalArticle

Nassar, AEF, Rusling, JF, Tominaga, M, Yanagimoto, J & Nakashima, N 1996, 'Electrochemistry of cast films of ferredoxin and lipid bilayers on electrodes', Journal of Electroanalytical Chemistry, vol. 416, no. 1-2, pp. 183-185. https://doi.org/10.1016/S0022-0728(96)01013-3
Nassar, Alaa Eldin F. ; Rusling, James F. ; Tominaga, Masato ; Yanagimoto, Junko ; Nakashima, Naotoshi. / Electrochemistry of cast films of ferredoxin and lipid bilayers on electrodes. In: Journal of Electroanalytical Chemistry. 1996 ; Vol. 416, No. 1-2. pp. 183-185.
@article{c4e9cace321b469abb61731b6a053879,
title = "Electrochemistry of cast films of ferredoxin and lipid bilayers on electrodes",
abstract = "Structural stability and electrochemistry of chlorella ferredoxin immobilized in a cast film of molecular bilayer membranes of a biological lipid, dimyristoylphosphatidylcholine (DMPC) on a quartz substrate or a pyrolytic graphite electrode were examined. A specular reflectance spectrum of the film showed that the [2Fe-2S] cluster in ferredoxin retains its original structure even in an air atmosphere. This indicates that the lipid bilayers provide suitable microenvironments for a stable immobilization of the protein. Cast films of ferredoxin + DMPC on a graphite electrode gave well-defined, nearly reversible redox waves with a formal potential of -0.59 V vs. Ag|AgCl, which is close to the reported value at promoter-modified electrodes, indicating that the electrochemical communication is from native ferredoxin.",
author = "Nassar, {Alaa Eldin F.} and Rusling, {James F.} and Masato Tominaga and Junko Yanagimoto and Naotoshi Nakashima",
year = "1996",
month = "11",
day = "1",
doi = "10.1016/S0022-0728(96)01013-3",
language = "English",
volume = "416",
pages = "183--185",
journal = "Journal of Electroanalytical Chemistry",
issn = "0368-1874",
publisher = "Elsevier Sequoia",
number = "1-2",

}

TY - JOUR

T1 - Electrochemistry of cast films of ferredoxin and lipid bilayers on electrodes

AU - Nassar, Alaa Eldin F.

AU - Rusling, James F.

AU - Tominaga, Masato

AU - Yanagimoto, Junko

AU - Nakashima, Naotoshi

PY - 1996/11/1

Y1 - 1996/11/1

N2 - Structural stability and electrochemistry of chlorella ferredoxin immobilized in a cast film of molecular bilayer membranes of a biological lipid, dimyristoylphosphatidylcholine (DMPC) on a quartz substrate or a pyrolytic graphite electrode were examined. A specular reflectance spectrum of the film showed that the [2Fe-2S] cluster in ferredoxin retains its original structure even in an air atmosphere. This indicates that the lipid bilayers provide suitable microenvironments for a stable immobilization of the protein. Cast films of ferredoxin + DMPC on a graphite electrode gave well-defined, nearly reversible redox waves with a formal potential of -0.59 V vs. Ag|AgCl, which is close to the reported value at promoter-modified electrodes, indicating that the electrochemical communication is from native ferredoxin.

AB - Structural stability and electrochemistry of chlorella ferredoxin immobilized in a cast film of molecular bilayer membranes of a biological lipid, dimyristoylphosphatidylcholine (DMPC) on a quartz substrate or a pyrolytic graphite electrode were examined. A specular reflectance spectrum of the film showed that the [2Fe-2S] cluster in ferredoxin retains its original structure even in an air atmosphere. This indicates that the lipid bilayers provide suitable microenvironments for a stable immobilization of the protein. Cast films of ferredoxin + DMPC on a graphite electrode gave well-defined, nearly reversible redox waves with a formal potential of -0.59 V vs. Ag|AgCl, which is close to the reported value at promoter-modified electrodes, indicating that the electrochemical communication is from native ferredoxin.

UR - http://www.scopus.com/inward/record.url?scp=0030291295&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030291295&partnerID=8YFLogxK

U2 - 10.1016/S0022-0728(96)01013-3

DO - 10.1016/S0022-0728(96)01013-3

M3 - Article

AN - SCOPUS:0030291295

VL - 416

SP - 183

EP - 185

JO - Journal of Electroanalytical Chemistry

JF - Journal of Electroanalytical Chemistry

SN - 0368-1874

IS - 1-2

ER -