Electrokinetic preconcentration and highly stacking gel electrophoresis for analysis of high molecular weight protein complexes on a plastic microchip

Mohamad Reza Mohamadi, Laili Mahmoudian, Noritada Kaji, Manabu Tokeshi, Hiroshi Chuman, Yoshinobu Baba

Research output: Contribution to conferencePaper

Abstract

An efficient dynamic coating using methylcellulose and a non-ionic detergent (Tween-20) was developed which controlled protein adsorption onto the surface of microchannels on a microchip made of poly (methyl methacrylate), PMMA. Highly stacking microchip electrophoresis under non-denaturing condition was used for analyzing high molecular weight protein complexes on a plastic microchip. The method has the efficiency of 6*10 6 plate/m for a complex of mAb-HSA with MW of ∼220kDa and with the detection limit of few pM.

Original languageEnglish
Pages353-355
Number of pages3
Publication statusPublished - Jan 1 2006
Externally publishedYes
Event10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan
Duration: Nov 5 2006Nov 9 2006

Other

Other10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
CountryJapan
CityTokyo
Period11/5/0611/9/06

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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  • Cite this

    Mohamadi, M. R., Mahmoudian, L., Kaji, N., Tokeshi, M., Chuman, H., & Baba, Y. (2006). Electrokinetic preconcentration and highly stacking gel electrophoresis for analysis of high molecular weight protein complexes on a plastic microchip. 353-355. Paper presented at 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, Japan.