Electron microscopic studies of giant nucleus-like structure formed by λ DNA introduced into the cytoplasma of Xenopus laevis fertilized eggs and embryos

Koichiro Shiokawa, Kosuke Tashiro, K. Yamana, Munefumi Sameshima

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

When bacteriophage λ DNA was injected into the cytoplasm of the fertilized egg of Xenopus laevis, giant nucleus-like structures were assembled around the injected DNA. These nucleus-like structures survived during cleavage and were partitioned into blastomeres at the blastula stage. The nucleus-like structures formed in the uncleaved fertilized eggs and the blastula cells were both surrounded by a bilayer nuclear membrane with nuclear pore complexes. The ultrastructural features of the λ DNA-induced nucleus-like structure were considerably different from those of the normal blastula nucleus: although the nuclear pore complexes appeared to be normal, the 'nucleoplasm' was much too homogeneous as compared with that of the normal nucleus.

Original languageEnglish
Pages (from-to)253-261
Number of pages9
JournalCell Differentiation
Volume20
Issue number4
DOIs
Publication statusPublished - Jan 1 1987

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Blastula
Zygote
Xenopus laevis
Nuclear Pore
Embryonic Structures
Electrons
DNA
Blastomeres
Nuclear Envelope
Bacteriophages
Cytoplasm

All Science Journal Classification (ASJC) codes

  • Developmental Biology

Cite this

Electron microscopic studies of giant nucleus-like structure formed by λ DNA introduced into the cytoplasma of Xenopus laevis fertilized eggs and embryos. / Shiokawa, Koichiro; Tashiro, Kosuke; Yamana, K.; Sameshima, Munefumi.

In: Cell Differentiation, Vol. 20, No. 4, 01.01.1987, p. 253-261.

Research output: Contribution to journalArticle

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