Electron spin resonance detection of hydrogen peroxide as an endothelium-derived hyperpolarizing factor in porcine coronary microvessels

Tetsuya Matoba, Hiroaki Shimokawa, Keiko Morikawa, Hiroshi Kubota, Ikuko Kunihiro, Lemmy Urakami-Harasawa, Yasushi Mukai, Yoji Hirakawa, Takaaki Akaike, Akira Takeshita

Research output: Contribution to journalArticle

113 Citations (Scopus)

Abstract

Objective - Endothelium-derived hyperpolarizing factor (EDHF) plays an important role in modulating vascular tone, especially in microvessels, although its nature has yet to be elucidated. This study was designed to examine whether hydrogen peroxide (H2O2) is an EDHF in porcine coronary microvessels with use of an electron spin resonance (ESR) method to directly detect H2O2 production from the endothelium. Methods and Results - Isometric tension and membrane-potential recordings demonstrated that bradykinin and substance P caused EDHF-mediated relaxations and hyperpolarizations of porcine coronary microvessels in the presence of indomethacin and Nω-nitro-L-arginine. The contribution of H2O2 to the EDHF-mediated responses was demonstrated by the inhibitory effect of catalase and by the relaxing and hyperpolarizing effects of exogenous H2O2. Endothelial production of H2O2 was quantified in bradykinin- or substance P-stimulated intact blood vessels by ESR spectroscopy. Tiron, a superoxide scavenger that facilitates H2O2 formation, enhanced bradykinin-induced production of H2O2, as well as the EDHF-mediated relaxations and hyperpolarizations. By contrast, cytochrome P-450 inhibitors (sulfaphenazole or 17-octadecynoic acid) or a gap junction inhibitor (18α-glycyrrhetinic acid) failed to inhibit the EDHF-mediated relaxations. Involvement of endothelium-derived K+ was not evident in experiments with ouabain plus Ba2+ or exogenous K+. Conclusion - These results provide ESR evidence that H2O2 is an EDHF in porcine coronary microvessels.

Original languageEnglish
Pages (from-to)1224-1230
Number of pages7
JournalArteriosclerosis, thrombosis, and vascular biology
Volume23
Issue number7
DOIs
Publication statusPublished - Jul 1 2003

Fingerprint

Electron Spin Resonance Spectroscopy
Microvessels
Hydrogen Peroxide
Endothelium
Swine
Bradykinin
Substance P
Blood Vessels
Sulfaphenazole
1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt
Glycyrrhetinic Acid
Gap Junctions
Ouabain
Superoxides
Indomethacin
Membrane Potentials
Catalase
Arginine

All Science Journal Classification (ASJC) codes

  • Cardiology and Cardiovascular Medicine

Cite this

Electron spin resonance detection of hydrogen peroxide as an endothelium-derived hyperpolarizing factor in porcine coronary microvessels. / Matoba, Tetsuya; Shimokawa, Hiroaki; Morikawa, Keiko; Kubota, Hiroshi; Kunihiro, Ikuko; Urakami-Harasawa, Lemmy; Mukai, Yasushi; Hirakawa, Yoji; Akaike, Takaaki; Takeshita, Akira.

In: Arteriosclerosis, thrombosis, and vascular biology, Vol. 23, No. 7, 01.07.2003, p. 1224-1230.

Research output: Contribution to journalArticle

Matoba, T, Shimokawa, H, Morikawa, K, Kubota, H, Kunihiro, I, Urakami-Harasawa, L, Mukai, Y, Hirakawa, Y, Akaike, T & Takeshita, A 2003, 'Electron spin resonance detection of hydrogen peroxide as an endothelium-derived hyperpolarizing factor in porcine coronary microvessels', Arteriosclerosis, thrombosis, and vascular biology, vol. 23, no. 7, pp. 1224-1230. https://doi.org/10.1161/01.ATV.0000078601.79536.6C
Matoba, Tetsuya ; Shimokawa, Hiroaki ; Morikawa, Keiko ; Kubota, Hiroshi ; Kunihiro, Ikuko ; Urakami-Harasawa, Lemmy ; Mukai, Yasushi ; Hirakawa, Yoji ; Akaike, Takaaki ; Takeshita, Akira. / Electron spin resonance detection of hydrogen peroxide as an endothelium-derived hyperpolarizing factor in porcine coronary microvessels. In: Arteriosclerosis, thrombosis, and vascular biology. 2003 ; Vol. 23, No. 7. pp. 1224-1230.
@article{252613c33d93425fa4a3ba9f4b088c12,
title = "Electron spin resonance detection of hydrogen peroxide as an endothelium-derived hyperpolarizing factor in porcine coronary microvessels",
abstract = "Objective - Endothelium-derived hyperpolarizing factor (EDHF) plays an important role in modulating vascular tone, especially in microvessels, although its nature has yet to be elucidated. This study was designed to examine whether hydrogen peroxide (H2O2) is an EDHF in porcine coronary microvessels with use of an electron spin resonance (ESR) method to directly detect H2O2 production from the endothelium. Methods and Results - Isometric tension and membrane-potential recordings demonstrated that bradykinin and substance P caused EDHF-mediated relaxations and hyperpolarizations of porcine coronary microvessels in the presence of indomethacin and Nω-nitro-L-arginine. The contribution of H2O2 to the EDHF-mediated responses was demonstrated by the inhibitory effect of catalase and by the relaxing and hyperpolarizing effects of exogenous H2O2. Endothelial production of H2O2 was quantified in bradykinin- or substance P-stimulated intact blood vessels by ESR spectroscopy. Tiron, a superoxide scavenger that facilitates H2O2 formation, enhanced bradykinin-induced production of H2O2, as well as the EDHF-mediated relaxations and hyperpolarizations. By contrast, cytochrome P-450 inhibitors (sulfaphenazole or 17-octadecynoic acid) or a gap junction inhibitor (18α-glycyrrhetinic acid) failed to inhibit the EDHF-mediated relaxations. Involvement of endothelium-derived K+ was not evident in experiments with ouabain plus Ba2+ or exogenous K+. Conclusion - These results provide ESR evidence that H2O2 is an EDHF in porcine coronary microvessels.",
author = "Tetsuya Matoba and Hiroaki Shimokawa and Keiko Morikawa and Hiroshi Kubota and Ikuko Kunihiro and Lemmy Urakami-Harasawa and Yasushi Mukai and Yoji Hirakawa and Takaaki Akaike and Akira Takeshita",
year = "2003",
month = "7",
day = "1",
doi = "10.1161/01.ATV.0000078601.79536.6C",
language = "English",
volume = "23",
pages = "1224--1230",
journal = "Arteriosclerosis, Thrombosis, and Vascular Biology",
issn = "1079-5642",
publisher = "Lippincott Williams and Wilkins",
number = "7",

}

TY - JOUR

T1 - Electron spin resonance detection of hydrogen peroxide as an endothelium-derived hyperpolarizing factor in porcine coronary microvessels

AU - Matoba, Tetsuya

AU - Shimokawa, Hiroaki

AU - Morikawa, Keiko

AU - Kubota, Hiroshi

AU - Kunihiro, Ikuko

AU - Urakami-Harasawa, Lemmy

AU - Mukai, Yasushi

AU - Hirakawa, Yoji

AU - Akaike, Takaaki

AU - Takeshita, Akira

PY - 2003/7/1

Y1 - 2003/7/1

N2 - Objective - Endothelium-derived hyperpolarizing factor (EDHF) plays an important role in modulating vascular tone, especially in microvessels, although its nature has yet to be elucidated. This study was designed to examine whether hydrogen peroxide (H2O2) is an EDHF in porcine coronary microvessels with use of an electron spin resonance (ESR) method to directly detect H2O2 production from the endothelium. Methods and Results - Isometric tension and membrane-potential recordings demonstrated that bradykinin and substance P caused EDHF-mediated relaxations and hyperpolarizations of porcine coronary microvessels in the presence of indomethacin and Nω-nitro-L-arginine. The contribution of H2O2 to the EDHF-mediated responses was demonstrated by the inhibitory effect of catalase and by the relaxing and hyperpolarizing effects of exogenous H2O2. Endothelial production of H2O2 was quantified in bradykinin- or substance P-stimulated intact blood vessels by ESR spectroscopy. Tiron, a superoxide scavenger that facilitates H2O2 formation, enhanced bradykinin-induced production of H2O2, as well as the EDHF-mediated relaxations and hyperpolarizations. By contrast, cytochrome P-450 inhibitors (sulfaphenazole or 17-octadecynoic acid) or a gap junction inhibitor (18α-glycyrrhetinic acid) failed to inhibit the EDHF-mediated relaxations. Involvement of endothelium-derived K+ was not evident in experiments with ouabain plus Ba2+ or exogenous K+. Conclusion - These results provide ESR evidence that H2O2 is an EDHF in porcine coronary microvessels.

AB - Objective - Endothelium-derived hyperpolarizing factor (EDHF) plays an important role in modulating vascular tone, especially in microvessels, although its nature has yet to be elucidated. This study was designed to examine whether hydrogen peroxide (H2O2) is an EDHF in porcine coronary microvessels with use of an electron spin resonance (ESR) method to directly detect H2O2 production from the endothelium. Methods and Results - Isometric tension and membrane-potential recordings demonstrated that bradykinin and substance P caused EDHF-mediated relaxations and hyperpolarizations of porcine coronary microvessels in the presence of indomethacin and Nω-nitro-L-arginine. The contribution of H2O2 to the EDHF-mediated responses was demonstrated by the inhibitory effect of catalase and by the relaxing and hyperpolarizing effects of exogenous H2O2. Endothelial production of H2O2 was quantified in bradykinin- or substance P-stimulated intact blood vessels by ESR spectroscopy. Tiron, a superoxide scavenger that facilitates H2O2 formation, enhanced bradykinin-induced production of H2O2, as well as the EDHF-mediated relaxations and hyperpolarizations. By contrast, cytochrome P-450 inhibitors (sulfaphenazole or 17-octadecynoic acid) or a gap junction inhibitor (18α-glycyrrhetinic acid) failed to inhibit the EDHF-mediated relaxations. Involvement of endothelium-derived K+ was not evident in experiments with ouabain plus Ba2+ or exogenous K+. Conclusion - These results provide ESR evidence that H2O2 is an EDHF in porcine coronary microvessels.

UR - http://www.scopus.com/inward/record.url?scp=10744220399&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=10744220399&partnerID=8YFLogxK

U2 - 10.1161/01.ATV.0000078601.79536.6C

DO - 10.1161/01.ATV.0000078601.79536.6C

M3 - Article

VL - 23

SP - 1224

EP - 1230

JO - Arteriosclerosis, Thrombosis, and Vascular Biology

JF - Arteriosclerosis, Thrombosis, and Vascular Biology

SN - 1079-5642

IS - 7

ER -