Abstract
The mode of DNA-breaking actions of ascorbic acid (AsA) and triose reductone (TR) in the presence of Cu2+ was studied by use of 0.8% agarose slab gel electrophoretic analysis. The DNA-breaking actions of the mixture of AsA or TR and Cu2+ were inhibited by N2 gas, hydroxyl radical scavengers and catalase, indicating that the oxidation of AsA or TR by Cu2+ and the hydrogen peroxide and hydroxyl radicals resulting from the oxidation are essential for the fragmentation of DNA by the mixture of AsA or TR and Cu2+. However, the DNA-breaking activity of these reductones varied with pH, while their oxidation rates were proportional to the increase in pH. The marked fragmentation of DNA occurred at pH 4, 7 or 8. The breakages of DNA by AsA or TR in the presence of Cu2+ were concluded not to be associated with the oxidation rates of reductones.
| Original language | English |
|---|---|
| Pages (from-to) | 481-488 |
| Number of pages | 8 |
| Journal | Journal of Nutritional Science and Vitaminology |
| Volume | 29 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Jan 1 1983 |
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All Science Journal Classification (ASJC) codes
- Medicine (miscellaneous)
- Nutrition and Dietetics
Cite this
Electrophoretic Study on the DNA-Breaking Actions of Ascorbic Acid and Triose Reductone in the Presence of Cu2+. / Shinohara, Kazuki; So, Masayasu; Nonaka, Michiko; Nishiyama, Kazuo; Murakami, Hiroki; Omura, Hirohisa.
In: Journal of Nutritional Science and Vitaminology, Vol. 29, No. 4, 01.01.1983, p. 481-488.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Electrophoretic Study on the DNA-Breaking Actions of Ascorbic Acid and Triose Reductone in the Presence of Cu2+
AU - Shinohara, Kazuki
AU - So, Masayasu
AU - Nonaka, Michiko
AU - Nishiyama, Kazuo
AU - Murakami, Hiroki
AU - Omura, Hirohisa
PY - 1983/1/1
Y1 - 1983/1/1
N2 - The mode of DNA-breaking actions of ascorbic acid (AsA) and triose reductone (TR) in the presence of Cu2+ was studied by use of 0.8% agarose slab gel electrophoretic analysis. The DNA-breaking actions of the mixture of AsA or TR and Cu2+ were inhibited by N2 gas, hydroxyl radical scavengers and catalase, indicating that the oxidation of AsA or TR by Cu2+ and the hydrogen peroxide and hydroxyl radicals resulting from the oxidation are essential for the fragmentation of DNA by the mixture of AsA or TR and Cu2+. However, the DNA-breaking activity of these reductones varied with pH, while their oxidation rates were proportional to the increase in pH. The marked fragmentation of DNA occurred at pH 4, 7 or 8. The breakages of DNA by AsA or TR in the presence of Cu2+ were concluded not to be associated with the oxidation rates of reductones.
AB - The mode of DNA-breaking actions of ascorbic acid (AsA) and triose reductone (TR) in the presence of Cu2+ was studied by use of 0.8% agarose slab gel electrophoretic analysis. The DNA-breaking actions of the mixture of AsA or TR and Cu2+ were inhibited by N2 gas, hydroxyl radical scavengers and catalase, indicating that the oxidation of AsA or TR by Cu2+ and the hydrogen peroxide and hydroxyl radicals resulting from the oxidation are essential for the fragmentation of DNA by the mixture of AsA or TR and Cu2+. However, the DNA-breaking activity of these reductones varied with pH, while their oxidation rates were proportional to the increase in pH. The marked fragmentation of DNA occurred at pH 4, 7 or 8. The breakages of DNA by AsA or TR in the presence of Cu2+ were concluded not to be associated with the oxidation rates of reductones.
UR - http://www.scopus.com/inward/record.url?scp=0021044597&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021044597&partnerID=8YFLogxK
U2 - 10.3177/jnsv.29.481
DO - 10.3177/jnsv.29.481
M3 - Article
VL - 29
SP - 481
EP - 488
JO - Journal of Nutritional Science and Vitaminology
JF - Journal of Nutritional Science and Vitaminology
SN - 0301-4800
IS - 4
ER -