Electrophoretic Study on the DNA-Breaking Actions of Ascorbic Acid and Triose Reductone in the Presence of Cu2+

Kazuki Shinohara, Masayasu So, Michiko Nonaka, Kazuo Nishiyama, Hiroki Murakami, Hirohisa Omura

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The mode of DNA-breaking actions of ascorbic acid (AsA) and triose reductone (TR) in the presence of Cu2+ was studied by use of 0.8% agarose slab gel electrophoretic analysis. The DNA-breaking actions of the mixture of AsA or TR and Cu2+ were inhibited by N2 gas, hydroxyl radical scavengers and catalase, indicating that the oxidation of AsA or TR by Cu2+ and the hydrogen peroxide and hydroxyl radicals resulting from the oxidation are essential for the fragmentation of DNA by the mixture of AsA or TR and Cu2+. However, the DNA-breaking activity of these reductones varied with pH, while their oxidation rates were proportional to the increase in pH. The marked fragmentation of DNA occurred at pH 4, 7 or 8. The breakages of DNA by AsA or TR in the presence of Cu2+ were concluded not to be associated with the oxidation rates of reductones.

Original languageEnglish
Pages (from-to)481-488
Number of pages8
JournalJournal of Nutritional Science and Vitaminology
Volume29
Issue number4
DOIs
Publication statusPublished - 1983

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

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