Electrophoretic Study on the DNA-Breaking Actions of Ascorbic Acid and Triose Reductone in the Presence of Cu2+

Kazuki Shinohara; Masayasu So; Michiko Nonaka; Kazuo Nishiyama; Hiroki Murakami; Hirohisa Omura

doi:10.3177/jnsv.29.481

Electrophoretic Study on the DNA-Breaking Actions of Ascorbic Acid and Triose Reductone in the Presence of Cu2+

Kazuki Shinohara, Masayasu So, Michiko Nonaka, Kazuo Nishiyama, Hiroki Murakami, Hirohisa Omura

Food Science and Biotechnology

Research output: Contribution to journal › Article

19 Citations (Scopus)

Abstract

The mode of DNA-breaking actions of ascorbic acid (AsA) and triose reductone (TR) in the presence of Cu2+ was studied by use of 0.8% agarose slab gel electrophoretic analysis. The DNA-breaking actions of the mixture of AsA or TR and Cu2+ were inhibited by N2 gas, hydroxyl radical scavengers and catalase, indicating that the oxidation of AsA or TR by Cu2+ and the hydrogen peroxide and hydroxyl radicals resulting from the oxidation are essential for the fragmentation of DNA by the mixture of AsA or TR and Cu2+. However, the DNA-breaking activity of these reductones varied with pH, while their oxidation rates were proportional to the increase in pH. The marked fragmentation of DNA occurred at pH 4, 7 or 8. The breakages of DNA by AsA or TR in the presence of Cu2+ were concluded not to be associated with the oxidation rates of reductones.

Original language	English
Pages (from-to)	481-488
Number of pages	8
Journal	Journal of Nutritional Science and Vitaminology
Volume	29
Issue number	4
DOIs	https://doi.org/10.3177/jnsv.29.481
Publication status	Published - 1983

All Science Journal Classification (ASJC) codes

Medicine (miscellaneous)
Nutrition and Dietetics

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Shinohara, K., So, M., Nonaka, M., Nishiyama, K., Murakami, H., & Omura, H. (1983). Electrophoretic Study on the DNA-Breaking Actions of Ascorbic Acid and Triose Reductone in the Presence of Cu2+. Journal of Nutritional Science and Vitaminology, 29(4), 481-488. https://doi.org/10.3177/jnsv.29.481

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abstract = "The mode of DNA-breaking actions of ascorbic acid (AsA) and triose reductone (TR) in the presence of Cu2+ was studied by use of 0.8% agarose slab gel electrophoretic analysis. The DNA-breaking actions of the mixture of AsA or TR and Cu2+ were inhibited by N2 gas, hydroxyl radical scavengers and catalase, indicating that the oxidation of AsA or TR by Cu2+ and the hydrogen peroxide and hydroxyl radicals resulting from the oxidation are essential for the fragmentation of DNA by the mixture of AsA or TR and Cu2+. However, the DNA-breaking activity of these reductones varied with pH, while their oxidation rates were proportional to the increase in pH. The marked fragmentation of DNA occurred at pH 4, 7 or 8. The breakages of DNA by AsA or TR in the presence of Cu2+ were concluded not to be associated with the oxidation rates of reductones.",

author = "Kazuki Shinohara and Masayasu So and Michiko Nonaka and Kazuo Nishiyama and Hiroki Murakami and Hirohisa Omura",

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AU - Shinohara, Kazuki

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AU - Nishiyama, Kazuo

AU - Murakami, Hiroki

AU - Omura, Hirohisa

PY - 1983

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