Electrostatic effect on apparent kinetic parameters in a packed-bed immobilized enzyme reactor

Hiromitsu Miyakawa, Fumihide Shiraishi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The effect of electrostatic field on apparent kinetic parameters in a packed-bed immobilized enzyme reactor was investigated using a mathematical model. The relationship between the dimensionless concentration of substrate consumed in the reactor and the logarithm of the unconverted fraction of substrate at the reactor outlet was nonlinear and changed remarkably according to the magnitude of electrostatic potential and the sign of charges that carry the support and substrate. For the opposite sign of these charges, the apparent Michaelis constant was less than its intrinsic value in the region of high substrate concentration, which reflects a promoting effect on the enzyme reaction by the electrostatic attraction.

Original languageEnglish
Pages (from-to)589-590
Number of pages2
JournalKagaku Kogaku Ronbunshu
Volume23
Issue number4
Publication statusPublished - 1997
Externally publishedYes

Fingerprint

Immobilized Enzymes
Packed beds
Kinetic parameters
Electrostatics
Enzymes
Substrates
Electric fields
Mathematical models

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Electrostatic effect on apparent kinetic parameters in a packed-bed immobilized enzyme reactor. / Miyakawa, Hiromitsu; Shiraishi, Fumihide.

In: Kagaku Kogaku Ronbunshu, Vol. 23, No. 4, 1997, p. 589-590.

Research output: Contribution to journalArticle

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