Elevated IgG against the C region of streptococcal M protein and its immunodeterminants in patients with poststreptococcal acute glomerulonephritis

K. Mori, Y. Ito, N. Kamikawaji, Takehiko Sasazuki

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Objective: To determine the immune responses to the streptococcal M protein in patients with poststreptococcal acute glomerulonephritis (PSAGN). Study design: The gene coding type 12 M protein of group A streptococcus (M12), a known PSAGN-associated serotype, was cloned and expressed in Escherichia coli to investigate the specific immune responses to the M12 protein in patients with PSAGN. Recombinant M proteins for the variable N- terminal half (AB region) and conserved C-terminal half (G region) were produced separately. IgG titers against each region were measured by enzyme- linked immunosorbent assay in patients with PSAGN (n = 51), uncomplicated streptococcal pharyngitis (n = 26), chronic glomerulonephritis (n = 10), and in healthy control subjects (n = 51). Immunodominant domains within the M protein in PSAGN were further investigated by use of overlapping synthetic peptides. Results: IgG titers against the C region, but not the AB region, were markedly higher in the PSAGN group than in other groups (p < 0.01), and these titers were maintained for at least several months after antistreptolysin O or antistreptokinase levels had returned to normal. Studies with overlapping synthetic peptides demonstrated that increased IgG reactivity was observed against the C repeat blocks. Conclusion: IgG titers against the C region are significantly elevated in patients with PSAGN, and it may be a diagnostic marker for PSAGN.

Original languageEnglish
Pages (from-to)293-299
Number of pages7
JournalJournal of Pediatrics
Volume131
Issue number2
DOIs
Publication statusPublished - Jan 1 1997
Externally publishedYes

Fingerprint

Glomerulonephritis
Immunoglobulin G
Antistreptolysin
streptococcal M protein
Immunodominant Epitopes
Peptides
Proteins
Pharyngitis
Streptococcus
Recombinant Proteins
Healthy Volunteers
Enzyme-Linked Immunosorbent Assay
Escherichia coli

All Science Journal Classification (ASJC) codes

  • Pediatrics, Perinatology, and Child Health

Cite this

Elevated IgG against the C region of streptococcal M protein and its immunodeterminants in patients with poststreptococcal acute glomerulonephritis. / Mori, K.; Ito, Y.; Kamikawaji, N.; Sasazuki, Takehiko.

In: Journal of Pediatrics, Vol. 131, No. 2, 01.01.1997, p. 293-299.

Research output: Contribution to journalArticle

@article{6261d4444d2444e4842cf36a85b3a442,
title = "Elevated IgG against the C region of streptococcal M protein and its immunodeterminants in patients with poststreptococcal acute glomerulonephritis",
abstract = "Objective: To determine the immune responses to the streptococcal M protein in patients with poststreptococcal acute glomerulonephritis (PSAGN). Study design: The gene coding type 12 M protein of group A streptococcus (M12), a known PSAGN-associated serotype, was cloned and expressed in Escherichia coli to investigate the specific immune responses to the M12 protein in patients with PSAGN. Recombinant M proteins for the variable N- terminal half (AB region) and conserved C-terminal half (G region) were produced separately. IgG titers against each region were measured by enzyme- linked immunosorbent assay in patients with PSAGN (n = 51), uncomplicated streptococcal pharyngitis (n = 26), chronic glomerulonephritis (n = 10), and in healthy control subjects (n = 51). Immunodominant domains within the M protein in PSAGN were further investigated by use of overlapping synthetic peptides. Results: IgG titers against the C region, but not the AB region, were markedly higher in the PSAGN group than in other groups (p < 0.01), and these titers were maintained for at least several months after antistreptolysin O or antistreptokinase levels had returned to normal. Studies with overlapping synthetic peptides demonstrated that increased IgG reactivity was observed against the C repeat blocks. Conclusion: IgG titers against the C region are significantly elevated in patients with PSAGN, and it may be a diagnostic marker for PSAGN.",
author = "K. Mori and Y. Ito and N. Kamikawaji and Takehiko Sasazuki",
year = "1997",
month = "1",
day = "1",
doi = "10.1016/S0022-3476(97)70169-0",
language = "English",
volume = "131",
pages = "293--299",
journal = "Journal of Pediatrics",
issn = "0022-3476",
publisher = "Mosby Inc.",
number = "2",

}

TY - JOUR

T1 - Elevated IgG against the C region of streptococcal M protein and its immunodeterminants in patients with poststreptococcal acute glomerulonephritis

AU - Mori, K.

AU - Ito, Y.

AU - Kamikawaji, N.

AU - Sasazuki, Takehiko

PY - 1997/1/1

Y1 - 1997/1/1

N2 - Objective: To determine the immune responses to the streptococcal M protein in patients with poststreptococcal acute glomerulonephritis (PSAGN). Study design: The gene coding type 12 M protein of group A streptococcus (M12), a known PSAGN-associated serotype, was cloned and expressed in Escherichia coli to investigate the specific immune responses to the M12 protein in patients with PSAGN. Recombinant M proteins for the variable N- terminal half (AB region) and conserved C-terminal half (G region) were produced separately. IgG titers against each region were measured by enzyme- linked immunosorbent assay in patients with PSAGN (n = 51), uncomplicated streptococcal pharyngitis (n = 26), chronic glomerulonephritis (n = 10), and in healthy control subjects (n = 51). Immunodominant domains within the M protein in PSAGN were further investigated by use of overlapping synthetic peptides. Results: IgG titers against the C region, but not the AB region, were markedly higher in the PSAGN group than in other groups (p < 0.01), and these titers were maintained for at least several months after antistreptolysin O or antistreptokinase levels had returned to normal. Studies with overlapping synthetic peptides demonstrated that increased IgG reactivity was observed against the C repeat blocks. Conclusion: IgG titers against the C region are significantly elevated in patients with PSAGN, and it may be a diagnostic marker for PSAGN.

AB - Objective: To determine the immune responses to the streptococcal M protein in patients with poststreptococcal acute glomerulonephritis (PSAGN). Study design: The gene coding type 12 M protein of group A streptococcus (M12), a known PSAGN-associated serotype, was cloned and expressed in Escherichia coli to investigate the specific immune responses to the M12 protein in patients with PSAGN. Recombinant M proteins for the variable N- terminal half (AB region) and conserved C-terminal half (G region) were produced separately. IgG titers against each region were measured by enzyme- linked immunosorbent assay in patients with PSAGN (n = 51), uncomplicated streptococcal pharyngitis (n = 26), chronic glomerulonephritis (n = 10), and in healthy control subjects (n = 51). Immunodominant domains within the M protein in PSAGN were further investigated by use of overlapping synthetic peptides. Results: IgG titers against the C region, but not the AB region, were markedly higher in the PSAGN group than in other groups (p < 0.01), and these titers were maintained for at least several months after antistreptolysin O or antistreptokinase levels had returned to normal. Studies with overlapping synthetic peptides demonstrated that increased IgG reactivity was observed against the C repeat blocks. Conclusion: IgG titers against the C region are significantly elevated in patients with PSAGN, and it may be a diagnostic marker for PSAGN.

UR - http://www.scopus.com/inward/record.url?scp=0030611930&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030611930&partnerID=8YFLogxK

U2 - 10.1016/S0022-3476(97)70169-0

DO - 10.1016/S0022-3476(97)70169-0

M3 - Article

C2 - 9290619

AN - SCOPUS:0030611930

VL - 131

SP - 293

EP - 299

JO - Journal of Pediatrics

JF - Journal of Pediatrics

SN - 0022-3476

IS - 2

ER -