Abstract
Objective: To determine the immune responses to the streptococcal M protein in patients with poststreptococcal acute glomerulonephritis (PSAGN). Study design: The gene coding type 12 M protein of group A streptococcus (M12), a known PSAGN-associated serotype, was cloned and expressed in Escherichia coli to investigate the specific immune responses to the M12 protein in patients with PSAGN. Recombinant M proteins for the variable N- terminal half (AB region) and conserved C-terminal half (G region) were produced separately. IgG titers against each region were measured by enzyme- linked immunosorbent assay in patients with PSAGN (n = 51), uncomplicated streptococcal pharyngitis (n = 26), chronic glomerulonephritis (n = 10), and in healthy control subjects (n = 51). Immunodominant domains within the M protein in PSAGN were further investigated by use of overlapping synthetic peptides. Results: IgG titers against the C region, but not the AB region, were markedly higher in the PSAGN group than in other groups (p < 0.01), and these titers were maintained for at least several months after antistreptolysin O or antistreptokinase levels had returned to normal. Studies with overlapping synthetic peptides demonstrated that increased IgG reactivity was observed against the C repeat blocks. Conclusion: IgG titers against the C region are significantly elevated in patients with PSAGN, and it may be a diagnostic marker for PSAGN.
| Original language | English |
|---|---|
| Pages (from-to) | 293-299 |
| Number of pages | 7 |
| Journal | Journal of Pediatrics |
| Volume | 131 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Jan 1 1997 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Pediatrics, Perinatology, and Child Health
Cite this
Elevated IgG against the C region of streptococcal M protein and its immunodeterminants in patients with poststreptococcal acute glomerulonephritis. / Mori, K.; Ito, Y.; Kamikawaji, N.; Sasazuki, Takehiko.
In: Journal of Pediatrics, Vol. 131, No. 2, 01.01.1997, p. 293-299.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Elevated IgG against the C region of streptococcal M protein and its immunodeterminants in patients with poststreptococcal acute glomerulonephritis
AU - Mori, K.
AU - Ito, Y.
AU - Kamikawaji, N.
AU - Sasazuki, Takehiko
PY - 1997/1/1
Y1 - 1997/1/1
N2 - Objective: To determine the immune responses to the streptococcal M protein in patients with poststreptococcal acute glomerulonephritis (PSAGN). Study design: The gene coding type 12 M protein of group A streptococcus (M12), a known PSAGN-associated serotype, was cloned and expressed in Escherichia coli to investigate the specific immune responses to the M12 protein in patients with PSAGN. Recombinant M proteins for the variable N- terminal half (AB region) and conserved C-terminal half (G region) were produced separately. IgG titers against each region were measured by enzyme- linked immunosorbent assay in patients with PSAGN (n = 51), uncomplicated streptococcal pharyngitis (n = 26), chronic glomerulonephritis (n = 10), and in healthy control subjects (n = 51). Immunodominant domains within the M protein in PSAGN were further investigated by use of overlapping synthetic peptides. Results: IgG titers against the C region, but not the AB region, were markedly higher in the PSAGN group than in other groups (p < 0.01), and these titers were maintained for at least several months after antistreptolysin O or antistreptokinase levels had returned to normal. Studies with overlapping synthetic peptides demonstrated that increased IgG reactivity was observed against the C repeat blocks. Conclusion: IgG titers against the C region are significantly elevated in patients with PSAGN, and it may be a diagnostic marker for PSAGN.
AB - Objective: To determine the immune responses to the streptococcal M protein in patients with poststreptococcal acute glomerulonephritis (PSAGN). Study design: The gene coding type 12 M protein of group A streptococcus (M12), a known PSAGN-associated serotype, was cloned and expressed in Escherichia coli to investigate the specific immune responses to the M12 protein in patients with PSAGN. Recombinant M proteins for the variable N- terminal half (AB region) and conserved C-terminal half (G region) were produced separately. IgG titers against each region were measured by enzyme- linked immunosorbent assay in patients with PSAGN (n = 51), uncomplicated streptococcal pharyngitis (n = 26), chronic glomerulonephritis (n = 10), and in healthy control subjects (n = 51). Immunodominant domains within the M protein in PSAGN were further investigated by use of overlapping synthetic peptides. Results: IgG titers against the C region, but not the AB region, were markedly higher in the PSAGN group than in other groups (p < 0.01), and these titers were maintained for at least several months after antistreptolysin O or antistreptokinase levels had returned to normal. Studies with overlapping synthetic peptides demonstrated that increased IgG reactivity was observed against the C repeat blocks. Conclusion: IgG titers against the C region are significantly elevated in patients with PSAGN, and it may be a diagnostic marker for PSAGN.
UR - http://www.scopus.com/inward/record.url?scp=0030611930&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030611930&partnerID=8YFLogxK
U2 - 10.1016/S0022-3476(97)70169-0
DO - 10.1016/S0022-3476(97)70169-0
M3 - Article
C2 - 9290619
AN - SCOPUS:0030611930
VL - 131
SP - 293
EP - 299
JO - Journal of Pediatrics
JF - Journal of Pediatrics
SN - 0022-3476
IS - 2
ER -