TY - JOUR
T1 - Elevation of intracellular calcium induced by the intrapipette perfusion technique modifies membrane ion currents in the chick cochlear hair cell
AU - Kimitsuki, Takashi
AU - Nakagawa, Takashi
AU - Hisashi, Kazutaka
AU - Tsuji, Kohji
AU - Komune, Sizuo
AU - Komiyama, Sohtaro
N1 - Funding Information:
This study was supported by a Grant-in-Aid for Scientific Research (08457453) from the Ministry of Education, Science and Culture of Japan.
PY - 1998
Y1 - 1998
N2 - The concentration of intracellular calcium ion ([Ca2+](i)) was altered in the same hair cell dissociated from a chick cochlea by the intrapipette perfusion technique. At a membrane potential of -40 mV, the elevation of [Ca2+](i) generated outward-going currents within 60 sec when the intrapipette solution was based on KCl. In controls, at membrane potentials more positive than -50 mV, outward K+ currents were observed and at large positive potentials, the outward K+ current decreased, showing an N-shaped I-V relationship. This outward K+ current was increased by elevation of [Ca2+](i) and was partially suppressed by a TEA-containing extracellular solution. We suggest that the Ca2+ increased by the intrapipette perfusion technique operates directly inside the cell membrane and activates Ca2+- activated K+ currents.
AB - The concentration of intracellular calcium ion ([Ca2+](i)) was altered in the same hair cell dissociated from a chick cochlea by the intrapipette perfusion technique. At a membrane potential of -40 mV, the elevation of [Ca2+](i) generated outward-going currents within 60 sec when the intrapipette solution was based on KCl. In controls, at membrane potentials more positive than -50 mV, outward K+ currents were observed and at large positive potentials, the outward K+ current decreased, showing an N-shaped I-V relationship. This outward K+ current was increased by elevation of [Ca2+](i) and was partially suppressed by a TEA-containing extracellular solution. We suggest that the Ca2+ increased by the intrapipette perfusion technique operates directly inside the cell membrane and activates Ca2+- activated K+ currents.
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U2 - 10.1080/00016489850155152
DO - 10.1080/00016489850155152
M3 - Article
C2 - 9504166
AN - SCOPUS:0031909802
VL - 118
SP - 70
EP - 73
JO - Acta Oto-Laryngologica
JF - Acta Oto-Laryngologica
SN - 0001-6489
IS - 1
ER -