Elevation of intracellular calcium induced by the intrapipette perfusion technique modifies membrane ion currents in the chick cochlear hair cell

Takashi Kimitsuki, Takashi Nakagawa, Kazutaka Hisashi, Kohji Tsuji, Sizuo Komune, Sohtaro Komiyama

Research output: Contribution to journalArticle

Abstract

The concentration of intracellular calcium ion ([Ca2+](i)) was altered in the same hair cell dissociated from a chick cochlea by the intrapipette perfusion technique. At a membrane potential of -40 mV, the elevation of [Ca2+](i) generated outward-going currents within 60 sec when the intrapipette solution was based on KCl. In controls, at membrane potentials more positive than -50 mV, outward K+ currents were observed and at large positive potentials, the outward K+ current decreased, showing an N-shaped I-V relationship. This outward K+ current was increased by elevation of [Ca2+](i) and was partially suppressed by a TEA-containing extracellular solution. We suggest that the Ca2+ increased by the intrapipette perfusion technique operates directly inside the cell membrane and activates Ca2+- activated K+ currents.

Original languageEnglish
Pages (from-to)70-73
Number of pages4
JournalActa Oto-Laryngologica
Volume118
Issue number1
DOIs
Publication statusPublished - Mar 18 1998

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Auditory Hair Cells
Membrane Potentials
Perfusion
Ions
Calcium
Membranes
Cochlea
Cell Membrane

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology

Cite this

Elevation of intracellular calcium induced by the intrapipette perfusion technique modifies membrane ion currents in the chick cochlear hair cell. / Kimitsuki, Takashi; Nakagawa, Takashi; Hisashi, Kazutaka; Tsuji, Kohji; Komune, Sizuo; Komiyama, Sohtaro.

In: Acta Oto-Laryngologica, Vol. 118, No. 1, 18.03.1998, p. 70-73.

Research output: Contribution to journalArticle

Kimitsuki, Takashi ; Nakagawa, Takashi ; Hisashi, Kazutaka ; Tsuji, Kohji ; Komune, Sizuo ; Komiyama, Sohtaro. / Elevation of intracellular calcium induced by the intrapipette perfusion technique modifies membrane ion currents in the chick cochlear hair cell. In: Acta Oto-Laryngologica. 1998 ; Vol. 118, No. 1. pp. 70-73.
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