TY - JOUR
T1 - ELISA for the Detection of Marker Compound for Crop Fertilizer Use of Various Medicinal Crop Extracts using Bacterium
AU - Son, Tae Kwon
AU - Eguchi, Toshihiko
AU - Shoyama, Yukihiro
AU - Tanaka, Hiroyuki
N1 - Publisher Copyright:
© 2018 Kyushu University. All rights reserved.
PY - 2019
Y1 - 2019
N2 - This study builds an easy and quick method of analysing the active ingredients in bio–fertilizers to ensure the uniformity of materials in bio–fertilizers that are made using ingredients that are cruder herbal materials or remnants after the primary medicinal crops are used as herbal materials. An ELISA was created using MAb, and it can substitute for HPLC in the analysis of saikosaponin a (SSa), ginsenoside (G–Rb1) and ginsenoside (G–Rg1), which are active ingredients of ginseng, and the ELISA can be used for various ginseng plants grown as typical medicinal crops. The concentration measurement ranges of G–Rb1, G–Rg1, and SSa are 12.5~250 ng/mL, 0.5~12.5 μg/mL, and 0.26~1.5 μg/mL, respectively.
AB - This study builds an easy and quick method of analysing the active ingredients in bio–fertilizers to ensure the uniformity of materials in bio–fertilizers that are made using ingredients that are cruder herbal materials or remnants after the primary medicinal crops are used as herbal materials. An ELISA was created using MAb, and it can substitute for HPLC in the analysis of saikosaponin a (SSa), ginsenoside (G–Rb1) and ginsenoside (G–Rg1), which are active ingredients of ginseng, and the ELISA can be used for various ginseng plants grown as typical medicinal crops. The concentration measurement ranges of G–Rb1, G–Rg1, and SSa are 12.5~250 ng/mL, 0.5~12.5 μg/mL, and 0.26~1.5 μg/mL, respectively.
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U2 - 10.5109/2231630
DO - 10.5109/2231630
M3 - Article
AN - SCOPUS:85132008734
SN - 0023-6152
VL - 64
SP - 27
EP - 32
JO - Journal of the Faculty of Agriculture, Kyushu University
JF - Journal of the Faculty of Agriculture, Kyushu University
IS - 1
ER -
