ELISA, western blotting, immunocytolocalization and immunoaffinity column for naturally occuring bioactive compounds using monoclonal antibodies

Shao Jic Shan, Waraporn Putalun, Noriko Fukuda, Osamu Morinaga, Hiroyuki Tanaka, Yukihiro Shoyama

Research output: Contribution to journalReview article

2 Citations (Scopus)

Abstract

Anti-sennoside A (SA) and anti-glycyrrhizin (GC) MAbs were used for screening and cloning. And they were also used for ELISA. New western blotting method of determination for GC and ginsenoside was established that compounds separated by silica gel TLC were transferred to a polyvinylidene difluoride (PVDF) membrane. The membrane was treated with NaIO4 solution followed by BSA, resulting in a ginsenoside-BSA conjugate on the PVDF membrane which was stained by the general western blotting. Immunocytolocalization of GC in the fresh root of Glycyrrhiza species was conducted using anti-GC MAb after blotting to PVDF membrane. Immunoaffinity column chromatography using anti-ginsenoside Rb1 (GRb1)MAb performs better than previously published separation methods resulting in pure GRb1 from the crude extracts of ginseng. On the other hand, total solasodine glycosides were separated by an immunoaffinity column using a wide-cross reactive anti-solamargine (SM)MAb.

Original languageEnglish
Pages (from-to)258-269
Number of pages12
JournalJournal of Food and Drug Analysis
Volume8
Issue number4
Publication statusPublished - Dec 1 2000

Fingerprint

glycyrrhizin
Glycyrrhizic Acid
monoclonal antibodies
Western blotting
Western Blotting
Enzyme-Linked Immunosorbent Assay
Monoclonal Antibodies
enzyme-linked immunosorbent assay
Ginsenosides
Membranes
Glycyrrhiza
Panax
Silica Gel
silica gel
Glycosides
Complex Mixtures
glycosides
Chromatography
Organism Cloning
molecular cloning

All Science Journal Classification (ASJC) codes

  • Food Science
  • Pharmacology

Cite this

ELISA, western blotting, immunocytolocalization and immunoaffinity column for naturally occuring bioactive compounds using monoclonal antibodies. / Shan, Shao Jic; Putalun, Waraporn; Fukuda, Noriko; Morinaga, Osamu; Tanaka, Hiroyuki; Shoyama, Yukihiro.

In: Journal of Food and Drug Analysis, Vol. 8, No. 4, 01.12.2000, p. 258-269.

Research output: Contribution to journalReview article

Shan, Shao Jic ; Putalun, Waraporn ; Fukuda, Noriko ; Morinaga, Osamu ; Tanaka, Hiroyuki ; Shoyama, Yukihiro. / ELISA, western blotting, immunocytolocalization and immunoaffinity column for naturally occuring bioactive compounds using monoclonal antibodies. In: Journal of Food and Drug Analysis. 2000 ; Vol. 8, No. 4. pp. 258-269.
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AU - Putalun, Waraporn

AU - Fukuda, Noriko

AU - Morinaga, Osamu

AU - Tanaka, Hiroyuki

AU - Shoyama, Yukihiro

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