Elucidation of metal-ion accumulation induced by hydrogen bonds on protein surfaces by using porous lysozyme crystals containing RhIII ions as the model surfaces

Takafumi Ueno, Satoshi Abe, Tomomi Koshiyama, Takahiro Ohki, Tatsuo Hikage, Yoshihito Watanabe

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Metal-ion accumulation on protein surfaces is a crucial step in the initiation of small-metal clusters and the formation of inorganic materials in nature. This event is expected to control the nucleation, growth, and position of the materials. There remain many unknowns, as to how proteins affect the initial process at the atomic level, although multistep assembly processes of the materials formation by both native and model systems have been clarified at the macroscopic level. Herein the cooperative effects of amino acids and hydrogen bonds promoting metal accumulation reactions are clarified by using porous hen egg white lysozyme (HEWL) crystals containing RhIII ions, as model protein surfaces for the reactions. The experimental results reveal noteworthy implications for initiation of metal accumulation, which involve highly cooperative dynamics of amino acids and hydrogen bonds: i) Disruption of hydrogen bonds can induce conformational changes of amino-acid residues to capture RhIII ions. ii) Water molecules pre-organized by hydrogen bonds can stabilize RhIII coordination as aqua ligands. iii) Water molecules participating in hydrogen bonds with amino-acid residues can be replaced by RhIII ions to form polynuclear structures with the residues. iv) RhIII aqua complexes are retained on amino-acid residues through stabilizing hydrogen bonds even at low pH (≈2). These metal-protein interactions including hydrogen bonds may promote native metal accumulation reactions and also may be useful in the preparation of new inorganic materials that incorporate proteins.

Original languageEnglish
Pages (from-to)2730-2740
Number of pages11
JournalChemistry - A European Journal
Volume16
Issue number9
DOIs
Publication statusPublished - Mar 1 2010

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Muramidase
Metal ions
Hydrogen bonds
Membrane Proteins
Enzymes
Ions
Proteins
Amino acids
Crystals
Metals
Amino Acids
Molecules
Water
Nucleation
Ligands

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Organic Chemistry

Cite this

Elucidation of metal-ion accumulation induced by hydrogen bonds on protein surfaces by using porous lysozyme crystals containing RhIII ions as the model surfaces. / Ueno, Takafumi; Abe, Satoshi; Koshiyama, Tomomi; Ohki, Takahiro; Hikage, Tatsuo; Watanabe, Yoshihito.

In: Chemistry - A European Journal, Vol. 16, No. 9, 01.03.2010, p. 2730-2740.

Research output: Contribution to journalArticle

Ueno, Takafumi ; Abe, Satoshi ; Koshiyama, Tomomi ; Ohki, Takahiro ; Hikage, Tatsuo ; Watanabe, Yoshihito. / Elucidation of metal-ion accumulation induced by hydrogen bonds on protein surfaces by using porous lysozyme crystals containing RhIII ions as the model surfaces. In: Chemistry - A European Journal. 2010 ; Vol. 16, No. 9. pp. 2730-2740.
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