Elucidation of novel budding yeast separase mutants

Yoshihito Shimizu, Masayoshi Nagai, Akter M.S.T. Yeasmin, Naoki Koike, Muhammad Waliullah Talukdar, Takashi Ushimaru

Research output: Contribution to journalArticlepeer-review


The mitotic separase cleaves Scc1 in cohesin to allow sister chromatids to separate from each other upon anaphase onset. Separase is also required for DNA damage repair. Here, we isolated and characterized 10 temperature-sensitive (ts) mutants of separase ESP1 in the budding yeast Saccharomyces cerevisiae. All mutants were defective in sister chromatid separation at the restricted temperature. Some esp1-ts mutants were hypersensitive to the microtubule poison benomyl and/or the DNA-damaging agent bleomycin. Overexpression of securin alleviated the growth defect in some esp1-ts mutants, whereas it rather exacerbated it in others. The Drosophila Pumilio homolog MPT5 was isolated as a high-dosage suppressor of esp1-ts cells. We discuss various features of separase based on these findings.

Original languageEnglish
Pages (from-to)473-478
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Issue number3
Publication statusPublished - 2016
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry


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