Elucidation of novel budding yeast separase mutants

Yoshihito Shimizu; Masayoshi Nagai; Akter M.S.T. Yeasmin; Naoki Koike; Muhammad Waliullah Talukdar; Takashi Ushimaru

doi:10.1080/09168451.2015.1101337

Elucidation of novel budding yeast separase mutants

Yoshihito Shimizu, Masayoshi Nagai, Akter M.S.T. Yeasmin, Naoki Koike, Muhammad Waliullah Talukdar, Takashi Ushimaru

Research output: Contribution to journal › Article › peer-review

Abstract

The mitotic separase cleaves Scc1 in cohesin to allow sister chromatids to separate from each other upon anaphase onset. Separase is also required for DNA damage repair. Here, we isolated and characterized 10 temperature-sensitive (ts) mutants of separase ESP1 in the budding yeast Saccharomyces cerevisiae. All mutants were defective in sister chromatid separation at the restricted temperature. Some esp1-ts mutants were hypersensitive to the microtubule poison benomyl and/or the DNA-damaging agent bleomycin. Overexpression of securin alleviated the growth defect in some esp1-ts mutants, whereas it rather exacerbated it in others. The Drosophila Pumilio homolog MPT5 was isolated as a high-dosage suppressor of esp1-ts cells. We discuss various features of separase based on these findings.

Original language	English
Pages (from-to)	473-478
Number of pages	6
Journal	Bioscience, Biotechnology and Biochemistry
Volume	80
Issue number	3
DOIs	https://doi.org/10.1080/09168451.2015.1101337
Publication status	Published - 2016
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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10.1080/09168451.2015.1101337

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abstract = "The mitotic separase cleaves Scc1 in cohesin to allow sister chromatids to separate from each other upon anaphase onset. Separase is also required for DNA damage repair. Here, we isolated and characterized 10 temperature-sensitive (ts) mutants of separase ESP1 in the budding yeast Saccharomyces cerevisiae. All mutants were defective in sister chromatid separation at the restricted temperature. Some esp1-ts mutants were hypersensitive to the microtubule poison benomyl and/or the DNA-damaging agent bleomycin. Overexpression of securin alleviated the growth defect in some esp1-ts mutants, whereas it rather exacerbated it in others. The Drosophila Pumilio homolog MPT5 was isolated as a high-dosage suppressor of esp1-ts cells. We discuss various features of separase based on these findings.",

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AU - Shimizu, Yoshihito

AU - Nagai, Masayoshi

AU - Yeasmin, Akter M.S.T.

AU - Koike, Naoki

AU - Talukdar, Muhammad Waliullah

AU - Ushimaru, Takashi

PY - 2016

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Elucidation of novel budding yeast separase mutants

Abstract

All Science Journal Classification (ASJC) codes

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