Elucidation of the role of sugar chains in glucoamylase using endo-β-N-acetylglucosaminidase from Flavobacterium sp.

Kaoru Takegawa, Masaki Inami, Kenji Yamamoto, Hidehiko Kumagai, Tatsurokuro Tochikura, Bunzo Mikami, Yuhei Morita

Research output: Contribution to journalArticle

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Abstract

Endo-β-N-acetylglucosaminidase (glycopeptide-d-mannosyl-N4-(N-acetyl-d-glucosaminyl)2-asparagine1,4-N-acetyl-β-glucosaminohydrolase, EC 3.2.1.96), homogenized from the culture filtrate of Flavobacterium sp., could liberate about 50% of the sugar chains from the glucoamylase of Rhizopus niveus. The native and carbohydrate-depleted glucoamylases were compared in their various enzymatic properties. It was found that they were identical in their catalytic activities. However, the carbohydrate-depleted glucoamylase was less thermally stable than the native glucoamylase. Moreover, the carbohydrate-depleted glucoamylase was more sensitive to proteinases such as pronase, subtilisin and trypsin. These results suggest that the sugar chains of the glucoamylase contribute to the high stability of the enzyme. However, circular dichroism spectra of the native and carbohydrate-depleted glucoamylase were found to be identical.

Original languageEnglish
Pages (from-to)187-193
Number of pages7
JournalBiochimica et Biophysica Acta (BBA)/Protein Structure and Molecular
Volume955
Issue number2
DOIs
Publication statusPublished - Jul 20 1988

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Flavobacterium
Glucan 1,4-alpha-Glucosidase
Acetylglucosaminidase
Sugars
Carbohydrates
Subtilisin
Enzyme Stability
Rhizopus
Pronase
Glycopeptides
Circular Dichroism
endo-alpha-sialidase
Trypsin
Catalyst activity
Peptide Hydrolases
Enzymes

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Molecular Biology

Cite this

Elucidation of the role of sugar chains in glucoamylase using endo-β-N-acetylglucosaminidase from Flavobacterium sp. / Takegawa, Kaoru; Inami, Masaki; Yamamoto, Kenji; Kumagai, Hidehiko; Tochikura, Tatsurokuro; Mikami, Bunzo; Morita, Yuhei.

In: Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular, Vol. 955, No. 2, 20.07.1988, p. 187-193.

Research output: Contribution to journalArticle

Takegawa, Kaoru ; Inami, Masaki ; Yamamoto, Kenji ; Kumagai, Hidehiko ; Tochikura, Tatsurokuro ; Mikami, Bunzo ; Morita, Yuhei. / Elucidation of the role of sugar chains in glucoamylase using endo-β-N-acetylglucosaminidase from Flavobacterium sp. In: Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular. 1988 ; Vol. 955, No. 2. pp. 187-193.
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