Elucidation of thioredoxin target protein networks in mouse

Cexiong Fu, Changgong Wu, Tong Liu, Tetsuro Ago, Peiyong Zhai, Junichi Sadoshima, Hong Li

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Thioredoxin 1 (Trx1) is a key redox modulator that is functionally conserved across a wide range of species, including plants, bacteria, and mammals. Using a conserved CXXC motif, Trx1 catalyzes the reduction of cysteine disulfides and S-nitrosothiols. In contrast to small molecular reductants such as glutathione and cysteine that can reduce a wide range of oxidized proteins, Trx1 reduces only selected proteins via specific protein-protein interaction. Trx1 has been shown to regulate numerous signal transduction pathways, and its dysfunctions have been implicated in several diseases, including cancer, inflammation, and neurodegenerative and cardiovascular diseases. Identification of Trx1 target proteins may help to identify novel signaling mechanisms that are important for Trx1 antistress responses. In this study, we performed an ICAT proteomics study for the identification of Trx1 target proteins from the hearts of a cardiac specific Trx1-overexpressing transgenic mouse model (Tg-Trx1). Trx1-reduced proteins were distinguished from Trx1-induced proteins by comparison of the ICAT results with those obtained using a parallel iTRAQ (isobaric tags for relative and absolute quantitation) protein expression analysis. We were able to identify 78 putative Trx1 reductive sites in 55 proteins. Interestingly we identified a few protein functional networks that had not been shown previously to be regulated by Trx1, including the creatine-phosphocreatine shuttle, the mitochondrial permeability transition pore complex, and the cardiac contractile apparatus. The results presented here suggest that in addition to a general antioxidant function, Trx1 may be involved in the coordination of a wide array of cellular functions for maintaining proper cardiac energy dynamics and facilitating muscle contraction.

Original languageEnglish
Pages (from-to)1674-1687
Number of pages14
JournalMolecular and Cellular Proteomics
Volume8
Issue number7
DOIs
Publication statusPublished - Dec 8 2009

Fingerprint

Thioredoxins
Proteins
Cysteine
S-Nitrosothiols
Signal transduction
Mammals
Phosphocreatine
Creatine
Reducing Agents
Muscle Contraction
Disulfides
Neurodegenerative Diseases
Proteomics
Modulators
Transgenic Mice
Oxidation-Reduction
Glutathione
Muscle

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology

Cite this

Elucidation of thioredoxin target protein networks in mouse. / Fu, Cexiong; Wu, Changgong; Liu, Tong; Ago, Tetsuro; Zhai, Peiyong; Sadoshima, Junichi; Li, Hong.

In: Molecular and Cellular Proteomics, Vol. 8, No. 7, 08.12.2009, p. 1674-1687.

Research output: Contribution to journalArticle

Fu, Cexiong ; Wu, Changgong ; Liu, Tong ; Ago, Tetsuro ; Zhai, Peiyong ; Sadoshima, Junichi ; Li, Hong. / Elucidation of thioredoxin target protein networks in mouse. In: Molecular and Cellular Proteomics. 2009 ; Vol. 8, No. 7. pp. 1674-1687.
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