Embedding of individual ferritin molecules in large, self-supporting silica nanofilms

Shigenori Fujikawa, Emi Muto, Toyoki Kunitake

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We report herein the fabrication of ferritin-embedded self-supporting silica nanofilms via a simple spin-coating process. Ferritin was employed as a template molecule, and solutions of ferritin and silica were spread on a polymercoated silicon substrate, in this order, After dissolving the polymer underlayer by simply immersing ethanol, a centimeter-sized self-supporting nanofilm of ferritin/silica composite with a thickness of 15 nm was successfully transferred onto an alumina membrane without the film breaking. Ozone and hydrochloric acid solution treatment removed the template ferritin molecules from the composite film to produce corresponding transmembrane nanoholes. The reported method is very simple, and the fabrication of a protein-embedded self-supporting nanofilm enables the design of biomembranemimetic devices.

Original languageEnglish
Pages (from-to)4629-4633
Number of pages5
JournalLangmuir
Volume23
Issue number8
DOIs
Publication statusPublished - Apr 10 2007
Externally publishedYes

Fingerprint

Ferritins
Silicon Dioxide
embedding
Silica
silicon dioxide
Molecules
templates
Fabrication
molecules
fabrication
composite materials
hydrochloric acid
Spin coating
Composite films
Hydrochloric acid
Ozone
ozone
coating
dissolving
Ethanol

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

Cite this

Embedding of individual ferritin molecules in large, self-supporting silica nanofilms. / Fujikawa, Shigenori; Muto, Emi; Kunitake, Toyoki.

In: Langmuir, Vol. 23, No. 8, 10.04.2007, p. 4629-4633.

Research output: Contribution to journalArticle

Fujikawa, Shigenori ; Muto, Emi ; Kunitake, Toyoki. / Embedding of individual ferritin molecules in large, self-supporting silica nanofilms. In: Langmuir. 2007 ; Vol. 23, No. 8. pp. 4629-4633.
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