Embryonic hematopoietic progenitor cells reside in muscle before bone marrow hematopoiesis

Yuka Tanaka, Tomoko Inoue-Yokoo, Kasem Kulkeaw, Chiyo Yanagi-Mizuochi, Senji Shirasawa, Yoichi Nakanishi, Daisuke Sugiyama

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In mice, hematopoietic cells home to bone marrow from fetal liver prenatally. To elucidate mechanisms underlying homing, we performed immunohistochemistry with the hematopoietic cell marker c-Kit, and observed c-Kit(+) cells localized inside muscle surrounding bone after 14.5 days post coitum. Flow cytometric analysis showed that CD45(+) c-Kit(+) hematopoietic cells were more abundant in muscle than in bone marrow between 14.5 and 17.5 days post coitum, peaking at 16.5 days post coitum. CD45(+) c-Kit(+) cells in muscle at 16.5 days post coitum exhibited higher expression of Gata2, among several hematopoietic genes, than did fetal liver or bone marrow cells. Colony formation assays revealed that muscle hematopoietic cells possess hematopoietic progenitor activity. Furthermore, exo utero transplantation revealed that fetal liver hematopoietic progenitor cells home to muscle and then to BM. Our findings demonstrate that hematopoietic progenitor cell homing occurs earlier than previously reported and that hematopoietic progenitor cells reside in muscle tissue before bone marrow hematopoiesis occurs during mouse embryogenesis.

Original languageEnglish
Article numbere0138621
JournalPLoS One
Volume10
Issue number9
DOIs
Publication statusPublished - Sep 21 2015

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hematopoiesis
Hematopoiesis
Hematopoietic Stem Cells
bone marrow
Muscle
Bone
Bone Marrow
Muscles
muscles
Liver
Muscle Cells
liver
cells
bone marrow cells
mice
muscle tissues
Bone Marrow Cells
myocytes
Embryonic Development
immunohistochemistry

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Tanaka, Y., Inoue-Yokoo, T., Kulkeaw, K., Yanagi-Mizuochi, C., Shirasawa, S., Nakanishi, Y., & Sugiyama, D. (2015). Embryonic hematopoietic progenitor cells reside in muscle before bone marrow hematopoiesis. PLoS One, 10(9), [e0138621]. https://doi.org/10.1371/journal.pone.0138621

Embryonic hematopoietic progenitor cells reside in muscle before bone marrow hematopoiesis. / Tanaka, Yuka; Inoue-Yokoo, Tomoko; Kulkeaw, Kasem; Yanagi-Mizuochi, Chiyo; Shirasawa, Senji; Nakanishi, Yoichi; Sugiyama, Daisuke.

In: PLoS One, Vol. 10, No. 9, e0138621, 21.09.2015.

Research output: Contribution to journalArticle

Tanaka, Y, Inoue-Yokoo, T, Kulkeaw, K, Yanagi-Mizuochi, C, Shirasawa, S, Nakanishi, Y & Sugiyama, D 2015, 'Embryonic hematopoietic progenitor cells reside in muscle before bone marrow hematopoiesis', PLoS One, vol. 10, no. 9, e0138621. https://doi.org/10.1371/journal.pone.0138621
Tanaka Y, Inoue-Yokoo T, Kulkeaw K, Yanagi-Mizuochi C, Shirasawa S, Nakanishi Y et al. Embryonic hematopoietic progenitor cells reside in muscle before bone marrow hematopoiesis. PLoS One. 2015 Sep 21;10(9). e0138621. https://doi.org/10.1371/journal.pone.0138621
Tanaka, Yuka ; Inoue-Yokoo, Tomoko ; Kulkeaw, Kasem ; Yanagi-Mizuochi, Chiyo ; Shirasawa, Senji ; Nakanishi, Yoichi ; Sugiyama, Daisuke. / Embryonic hematopoietic progenitor cells reside in muscle before bone marrow hematopoiesis. In: PLoS One. 2015 ; Vol. 10, No. 9.
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