Embryonic undifferentiated cells show scattering activity on a surface coated with immobilized E-cadherin

Masato Nagaoka, Hirohiko Ise, Ichiro Harada, Uichi Koshimizu, Atsushi Maruyama, Toshihiro Akaike

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Rearrangement of cell-cell adhesion is a critical event in embryonic development and tissue formation. We investigated the regulatory function of E-cadherin, a key adhesion protein, in the developmental process by using E-cadherin/IgG Fc fusion protein as an adhesion matrix in cell culture. F9 embryonal carcinoma cells usually form colonies when cultured on gelatin or fibronectin matrices. However, F9 cells cultured on the E-cadherin/IgG Fc fusion protein matrix formed a scattered distribution, with a different cytoskeletal organization and E-cadherin-rich protrusions that were regulated by Rac1 activity. The same scattering activity was observed in P19 embryonal carcinoma cells. In contrast, three types of differentiated cells, NMuMG mammary gland cells, MDCK kidney epithelial cells, and mouse primary isolated hepatocytes, did not show the scattering activity observed in F9 and P19 cells. These results suggest that migratory behavior on an E-cadherin-immobilized surface is only observed in embryonic cells, and that the regulatory mechanisms underlying E-cadherin-mediated cell adhesion vary with the state of differentiation.

Original languageEnglish
Pages (from-to)296-310
Number of pages15
JournalJournal of Cellular Biochemistry
Volume103
Issue number1
DOIs
Publication statusPublished - Jan 1 2008
Externally publishedYes

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Cadherins
Scattering
Embryonal Carcinoma Stem Cells
Cell adhesion
Cell Adhesion
Fusion reactions
Adhesion
Immunoglobulin G
Cells
Proteins
Madin Darby Canine Kidney Cells
Gelatin
Human Mammary Glands
Fibronectins
Cell culture
Embryonic Development
Hepatocytes
Cultured Cells
Cell Culture Techniques
Epithelial Cells

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Embryonic undifferentiated cells show scattering activity on a surface coated with immobilized E-cadherin. / Nagaoka, Masato; Ise, Hirohiko; Harada, Ichiro; Koshimizu, Uichi; Maruyama, Atsushi; Akaike, Toshihiro.

In: Journal of Cellular Biochemistry, Vol. 103, No. 1, 01.01.2008, p. 296-310.

Research output: Contribution to journalArticle

Nagaoka, Masato ; Ise, Hirohiko ; Harada, Ichiro ; Koshimizu, Uichi ; Maruyama, Atsushi ; Akaike, Toshihiro. / Embryonic undifferentiated cells show scattering activity on a surface coated with immobilized E-cadherin. In: Journal of Cellular Biochemistry. 2008 ; Vol. 103, No. 1. pp. 296-310.
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