Emergence of a multidrug-resistant Shiga toxin-producing enterotoxigenic Escherichia coli lineage in diseased swine in Japan

Masahiro Kusumoto, Yuna Hikoda, Yuki Fujii, Misato Murata, Hirotsugu Miyoshi, Yoshitoshi Ogura, Yasuhiro Gotoh, Taketoshi Iwata, Tetsuya Hayashi, Masato Akiba

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Enterotoxigenic Escherichia coli (ETEC) and Shiga toxin-producing E. coli (STEC) are important causes of diarrhea and edema disease in swine. The majority of swine-pathogenic E. coli strains belong to a limited range of O serogroups, including O8, O138, O139, O141, O147, O149, and O157, which are the most frequently reported strains worldwide. However, the circumstances of ETEC and STEC infections in Japan remain unknown; there have been few reports on the prevalence or characterization of swine-pathogenic E. coli. In the present study, we determined the O serogroups of 967 E. coli isolates collected between 1991 and 2014 from diseased swine in Japan, and we found that O139, O149, O116, and OSB9 (O serogroup of Shigella boydii type 9) were the predominant serogroups. We further analyzed these four O serogroups using pulsed-field gel electrophoresis (PFGE), multilocus sequence typing, and virulence factor profiling. Most of the O139 and O149 strains formed serogroup-specific PFGE clusters (clusters I and II, respectively), whereas the O116 and OSB9 strains were grouped together in the same cluster (cluster III). All of the cluster III strains belonged to a single sequence type (ST88) and carried genes encoding both enterotoxin and Shiga toxin. This PFGE cluster III/ST88 lineage exhibited a high level of multidrug resistance (to a median of 10 antimicrobials). Notably, these bacteria were resistant to fluoroquinolones. Thus, this lineage should be considered a significant risk to animal production due to the toxigenicity and antimicrobial resistance of these bacteria.

Original languageEnglish
Pages (from-to)1074-1081
Number of pages8
JournalJournal of Clinical Microbiology
Volume54
Issue number4
DOIs
Publication statusPublished - Apr 2016

Fingerprint

Swine Diseases
Shiga-Toxigenic Escherichia coli
Enterotoxigenic Escherichia coli
Japan
Pulsed Field Gel Electrophoresis
Shiga Toxin
Escherichia coli
Edema Disease of Swine
Shigella boydii
Swine
Bacteria
Multilocus Sequence Typing
Escherichia coli Infections
Enterotoxins
Fluoroquinolones
Multiple Drug Resistance
Virulence Factors
Serogroup
Diarrhea

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

Cite this

Emergence of a multidrug-resistant Shiga toxin-producing enterotoxigenic Escherichia coli lineage in diseased swine in Japan. / Kusumoto, Masahiro; Hikoda, Yuna; Fujii, Yuki; Murata, Misato; Miyoshi, Hirotsugu; Ogura, Yoshitoshi; Gotoh, Yasuhiro; Iwata, Taketoshi; Hayashi, Tetsuya; Akiba, Masato.

In: Journal of Clinical Microbiology, Vol. 54, No. 4, 04.2016, p. 1074-1081.

Research output: Contribution to journalArticle

Kusumoto, Masahiro ; Hikoda, Yuna ; Fujii, Yuki ; Murata, Misato ; Miyoshi, Hirotsugu ; Ogura, Yoshitoshi ; Gotoh, Yasuhiro ; Iwata, Taketoshi ; Hayashi, Tetsuya ; Akiba, Masato. / Emergence of a multidrug-resistant Shiga toxin-producing enterotoxigenic Escherichia coli lineage in diseased swine in Japan. In: Journal of Clinical Microbiology. 2016 ; Vol. 54, No. 4. pp. 1074-1081.
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abstract = "Enterotoxigenic Escherichia coli (ETEC) and Shiga toxin-producing E. coli (STEC) are important causes of diarrhea and edema disease in swine. The majority of swine-pathogenic E. coli strains belong to a limited range of O serogroups, including O8, O138, O139, O141, O147, O149, and O157, which are the most frequently reported strains worldwide. However, the circumstances of ETEC and STEC infections in Japan remain unknown; there have been few reports on the prevalence or characterization of swine-pathogenic E. coli. In the present study, we determined the O serogroups of 967 E. coli isolates collected between 1991 and 2014 from diseased swine in Japan, and we found that O139, O149, O116, and OSB9 (O serogroup of Shigella boydii type 9) were the predominant serogroups. We further analyzed these four O serogroups using pulsed-field gel electrophoresis (PFGE), multilocus sequence typing, and virulence factor profiling. Most of the O139 and O149 strains formed serogroup-specific PFGE clusters (clusters I and II, respectively), whereas the O116 and OSB9 strains were grouped together in the same cluster (cluster III). All of the cluster III strains belonged to a single sequence type (ST88) and carried genes encoding both enterotoxin and Shiga toxin. This PFGE cluster III/ST88 lineage exhibited a high level of multidrug resistance (to a median of 10 antimicrobials). Notably, these bacteria were resistant to fluoroquinolones. Thus, this lineage should be considered a significant risk to animal production due to the toxigenicity and antimicrobial resistance of these bacteria.",
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AU - Miyoshi, Hirotsugu

AU - Ogura, Yoshitoshi

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AB - Enterotoxigenic Escherichia coli (ETEC) and Shiga toxin-producing E. coli (STEC) are important causes of diarrhea and edema disease in swine. The majority of swine-pathogenic E. coli strains belong to a limited range of O serogroups, including O8, O138, O139, O141, O147, O149, and O157, which are the most frequently reported strains worldwide. However, the circumstances of ETEC and STEC infections in Japan remain unknown; there have been few reports on the prevalence or characterization of swine-pathogenic E. coli. In the present study, we determined the O serogroups of 967 E. coli isolates collected between 1991 and 2014 from diseased swine in Japan, and we found that O139, O149, O116, and OSB9 (O serogroup of Shigella boydii type 9) were the predominant serogroups. We further analyzed these four O serogroups using pulsed-field gel electrophoresis (PFGE), multilocus sequence typing, and virulence factor profiling. Most of the O139 and O149 strains formed serogroup-specific PFGE clusters (clusters I and II, respectively), whereas the O116 and OSB9 strains were grouped together in the same cluster (cluster III). All of the cluster III strains belonged to a single sequence type (ST88) and carried genes encoding both enterotoxin and Shiga toxin. This PFGE cluster III/ST88 lineage exhibited a high level of multidrug resistance (to a median of 10 antimicrobials). Notably, these bacteria were resistant to fluoroquinolones. Thus, this lineage should be considered a significant risk to animal production due to the toxigenicity and antimicrobial resistance of these bacteria.

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